Nonaqueous capillary electrophoresis for the analysis of selected tropane alkaloids in a plant extract

被引：34

作者：

Cherkaoui, S ^{[1
]}

Mateus, L ^{[1
]}

Christen, P ^{[1
]}

Veuthey, JL ^{[1
]}

机构：

[1] Univ Geneva, Lab Pharmaceut Analyt Chem, CH-1211 Geneva 4, Switzerland

来源：

CHROMATOGRAPHIA | 1999年 / 49卷 / 1-2期

关键词：

capillary electrophoresis; micellar electrokinetic capillary chromatography; nonaqueous media; tropane alkaloids; hyoscyamine and scopolamine;

D O I：

10.1007/BF02467187

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential of nonaqueous capillary electrophoresis has been investigated for the separation of structurally similar tropane alkaloids. The effects of the organic solvent and of electrolyte composition on separation selectivity, migration times, and efficiency are described. The addition of trifluoroacetic acid to the separation buffer was found beneficial for manipulation of the order of migration of the two positional isomers littorine and hyoscyamine. Replicate injections under nonaqueous conditions gave migration time and peak area data of excellent precision. The application of the optimized conditions to the analysis of hyoscyamine and scopolamine in genetically transformed root cultures of Datura candida x D. aurea is presented.

引用

页码：54 / 60

页数：7

共 32 条

[1] Highly selective and efficient separations of a wide range of acidic species in capillary electrophoresis employing non-aqueous media
Altria, KD
Bryant, SM
[J]. CHROMATOGRAPHIA, 1997, 46 (3-4) : 122 - 130
[2] [Anonymous], [No title captured]
[3] BENSON LM, 1993, J HIGH RES CHROMATOG, V16, P324
[4] Determination of opium alkaloids in crude opium using non-aqueous capillary electrophoresis
Bjornsdottir, I
Hansen, SH
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1995, 13 (12) : 1473 - 1481
[5] COMPARISON OF SEPARATION SELECTIVITY IN AQUEOUS AND NONAQUEOUS CAPILLARY ELECTROPHORESIS
BJORNSDOTTIR, I
HANSEN, SH
[J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 711 (02) : 313 - 322
[6] Chiral separation in non-aqueous media by capillary electrophoresis using the ion-pair principle
Bjornsdottir, I
Hansen, SH
Terabe, S
[J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 745 (1-2) : 37 - 44
[7] Micellar electrokinetic capillary chromatography for selected tropane alkaloid analysis in plant extract
Cherkaoui, S
Mateus, L
Christen, P
Veuthey, JL
[J]. CHROMATOGRAPHIA, 1997, 46 (7-8) : 351 - 357
[8] Development and validation of a capillary zone electrophoresis method for the determination of atropine, homatropine and scopolamine in ophthalmic solutions
Cherkaoui, S
Mateus, L
Christen, P
Veuthey, JL
[J]. JOURNAL OF CHROMATOGRAPHY B, 1997, 696 (02): : 283 - 290
[9] CAPILLARY ZONE ELECTROPHORESIS IN ORGANIC-SOLVENTS - SEPARATION OF ANIONS IN METHANOLIC BUFFER SOLUTIONS
CHIARI, M
KENNDLER, E
[J]. JOURNAL OF CHROMATOGRAPHY A, 1995, 716 (1-2) : 303 - 309
[10] TROPANE ALKALOIDS IN TRANSFORMED ROOTS OF DATURA-QUERCIFOLIA
DUPRAZ, JM
CHRISTEN, P
KAPETANIDIS, I
[J]. PLANTA MEDICA, 1994, 60 (02) : 158 - 162

← 1 2 3 4 →