Cloning and sequencing of trehalose biosynthesis genes from Arthrobacter sp Q36

被引：60

作者：

Maruta, K

Hattori, K

Nakada, T

Kubota, M

Sugimoto, T

Kurimoto, M

机构：

[1] Hayashibara Biochem. Labs., Inc., Okayama 70

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1996年 / 1289卷 / 01期

关键词：

trehalose; maltooligosyltrehalose; gene cloning; nucleotide sequence; operon; (Arthrobacter);

D O I：

10.1016/0304-4165(95)00139-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A 5.1-kbp genomic DNA fragment was cloned from trehalose-producing bacterium Arthrobacter sp. strain Q36. Sequence analysis of the DNA fragment revealed two open reading frames of 2325 and 1794 bp, encoding maltooligosyltrehalose synthase (TreY) and maltooligosyltrehalose trehalohydrolase (TreZ), respectively. The 3' end of treY overlaps with the 5' end of treZ by one nucleotide, and it is suggested that treYZ constitutes an operon. The deduced amino acid sequences of both enzymes have several regions common to amylolytic enzymes belonging to an 'alpha-amylase family'.

引用

页码：10 / 13

页数：4

共 19 条

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