Transcriptional analysis of the tomato resistance response triggered by recognition of the Xanthomonas type III effector AvrXv3

被引:23
作者
Balaji, Vasudevan
Gibly, Avi
Debbie, Paul
Sessa, Guido [1 ]
机构
[1] Tel Aviv Univ, Dept Plant Sci, IL-69978 Tel Aviv, Israel
[2] Boyce Thompson Inst Plant Res, Ctr Gene Express Profiling, Ithaca, NY 14853 USA
关键词
AvrXv3; disease resistance; tomato; Xanthoinonas campestris pv. vesicatoria;
D O I
10.1007/s10142-007-0050-y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
The type III effector AvrXv3 from Xanthomonas campestris pv. vesicatoria (Xcv) elicits a resistance response in the tomato line Hawaii 7981. To test whether similar genes participate in responses triggered by recognition of different avirulence proteins, we examined the effect of AvrXv3 expression on the plant transcriptome as compared to that of other avirulence proteins. By microarray analysis we monitored expression of approximately 8,600 tomato genes upon inoculation with isogenic Xcv strains differing only by the avrXv3 gene. Changes in transcript levels of 139 genes were observed within 8 h, and a massive shift in expression of 1,294 genes was detected at 12 h. Recognition of AvrXv3 modulated a large number of genes encoding transcription factors and signaling components. In addition, genes involved in defense and stress responses, lipid metabolism, protein degradation, and secondary metabolism were mainly up-regulated. Conversely, genes related to photosynthesis and protein synthesis were generally down-regulated. Many novel genes encoding proteins of unknown function were also identified. A comparison between AvrXv3-modulated genes and those differentially expressed in tomato plants recognizing other bacteria] effectors revealed partial overlap and similar distribution in functional classes. The identification of tomato genes modulated by AvrXv3 expression paves the way for dissecting defense networks activated by recognition of this effector in resistant plants.
引用
收藏
页码:305 / 316
页数:12
相关论文
共 69 条
[1]   Bacterial elicitation and evasion of plant innate immunity [J].
Abramovitch, Robert B. ;
Anderson, Jeffrey C. ;
Martin, Gregory B. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2006, 7 (08) :601-611
[2]   ESTs, cDNA microarrays, and gene expression profiling: tools for dissecting plant physiology and development [J].
Alba, R ;
Fei, ZJ ;
Payton, P ;
Liu, Y ;
Moore, SL ;
Debbie, P ;
Cohn, J ;
D'Ascenzo, M ;
Gordon, JS ;
Rose, JKC ;
Martin, G ;
Tanksley, SD ;
Bouzayen, M ;
Jahn, MM ;
Giovannoni, J .
PLANT JOURNAL, 2004, 39 (05) :697-714
[3]   Oxylipin profiling of the hypersensitive response in Arabidopsis thaliana -: Formation of a novel oxo-phytodienoic acid-containing galactolipid, arabidopside E [J].
Andersson, Mats X. ;
Hamberg, Mats ;
Kourtchenko, Olga ;
Brunnstrom, Asa ;
McPhail, Kerry L. ;
Gerwick, William H. ;
Goebel, Cornelia ;
Feussner, Ivo ;
Ellerstrom, Mats .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (42) :31528-31537
[4]   Resistance of tomato and pepper to T3 strains of Xanthomonas campestris pv. vesicatoria is specified by a plant-inducible [J].
Astua-Monge, G ;
Minsavage, GV ;
Stall, RE ;
Davis, MJ ;
Bonas, U ;
Jones, JB .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2000, 13 (09) :911-921
[5]   Antimicrobial intermediates of the general phenylpropanoid and lignin specific pathways [J].
Barber, MS ;
McConnell, VS ;
DeCaux, BS .
PHYTOCHEMISTRY, 2000, 54 (01) :53-56
[6]  
Berrocal-Lobo M, 2004, MOL PLANT MICROBE IN, V17, P763, DOI 10.1094/MPMI.2004.17.7.763
[7]   Lignin biosynthesis [J].
Boerjan, W ;
Ralph, J ;
Baucher, M .
ANNUAL REVIEW OF PLANT BIOLOGY, 2003, 54 :519-546
[8]   Molecular properties of the Xanthomonas AvrRxv effector and global transcriptional changes determined by its expression in resistant tomato plants [J].
Bonshtien, A ;
Lev, A ;
Gibly, A ;
Debbie, P ;
Avni, A ;
Sessa, G .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2005, 18 (04) :300-310
[9]   Minimum information about a microarray experiment (MIAME) - toward standards for microarray data [J].
Brazma, A ;
Hingamp, P ;
Quackenbush, J ;
Sherlock, G ;
Spellman, P ;
Stoeckert, C ;
Aach, J ;
Ansorge, W ;
Ball, CA ;
Causton, HC ;
Gaasterland, T ;
Glenisson, P ;
Holstege, FCP ;
Kim, IF ;
Markowitz, V ;
Matese, JC ;
Parkinson, H ;
Robinson, A ;
Sarkans, U ;
Schulze-Kremer, S ;
Stewart, J ;
Taylor, R ;
Vilo, J ;
Vingron, M .
NATURE GENETICS, 2001, 29 (04) :365-371
[10]   Expression profile matrix of Arabidopsis transcription factor genes suggests their putative functions in response to environmental stresses [J].
Chen, WQ ;
Provart, NJ ;
Glazebrook, J ;
Katagiri, F ;
Chang, HS ;
Eulgem, T ;
Mauch, F ;
Luan, S ;
Zou, GZ ;
Whitham, SA ;
Budworth, PR ;
Tao, Y ;
Xie, ZY ;
Chen, X ;
Lam, S ;
Kreps, JA ;
Harper, JF ;
Si-Ammour, A ;
Mauch-Mani, B ;
Heinlein, M ;
Kobayashi, K ;
Hohn, T ;
Dangl, JL ;
Wang, X ;
Zhu, T .
PLANT CELL, 2002, 14 (03) :559-574