Effect of benzyladenine and indolebutyric acid on ultrastructure, glands formation, and essential oil accumulation in Lavandula dentata plantlets

被引:36
作者
Sudriá, C [1 ]
Palazón, J [1 ]
Cusidó, R [1 ]
Bonfill, M [1 ]
Piñol, MT [1 ]
Morales, C [1 ]
机构
[1] Univ Barcelona, Fac Pharm, Plant Physiol Lab, E-08028 Barcelona, Spain
关键词
axillary buds; chloroplasts; growth regulators; lavandin;
D O I
10.1023/A:1017998800224
中图分类号
Q94 [植物学];
学科分类号
071001 [植物学];
摘要
Lavandin (Lavandula dentata) axillary buds were grown in Linsmaier-Skoog (LS) medium solidified with 10 % bactoagar (control) and supplemented with 0.1 mg dm(-3) benzyladenine (BA), 0.1 mg dm(-3) indolebutyric acid (IBA) or both plant growth regulators. In the studied conditions the axillary buds developed into plantlets. The addition of BA inhibited the formation of glands by 44 % as compared with the control plantlets and also inhibited their development: these plantlets had the highest number of unbroken glands tin pre-secretory state) when compared with plantlets grown in the other conditions. The presence of BA stimulated chloroplast formation, and increased the content of essential oils by 150 % with respect to the control plantlets. It also increased their secretion, and the number of lipid droplets in the chloroplasts, cytosol and plasmalemma. On the contrary, the presence of IBA decreased the essential oil concentration in plantlets by 31 % when compared with the control ones and inhibited their secretion capacity.
引用
收藏
页码:1 / 6
页数:6
相关论文
共 22 条
[1]
BAJAJ YPS, 1988, BIOTECHNOLOGY AGR FO, V4, P60
[2]
BHATNAGAR JK, 1963, AM J PHARM, V165, P288
[3]
[4]
BRUNELLE B, 1983, SOFTWARE REV, V2, P245
[5]
CHARLWOOD BV, 1989, BIOFLAVOUR 87, P303
[6]
CROTEAU R, 1981, TERPENOIDS, P225
[7]
Croteau R., 1984, ISOPENTENOIDS PLANTS, P31
[8]
Davies P.J., 1987, PLANT HORMONES THEIR
[9]
GAMEZ MJ, 1990, PHARMAZIE, V45, P69
[10]
Goldthwaite J. J., 1987, Plant hormones and their role in plant growth and development., P553