Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain

被引：22

作者：

Zhanga, CH

Lu, JH

Wang, YF

Zheng, HY

Xiong, S

Zhang, MY

Liu, XJ

Li, JX

Wan, ZY

Yan, XG

Qi, SY

Cui, ZY

Zhang, BL

机构：

[1] Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou Int Business Incubator, Guangzhou 510663, Peoples R China

[2] Jinan Univ, Biomed Res & Dev Ctr, Guangzhou 510632, Peoples R China

[3] W Anhui Univ, Luan 237012, Peoples R China

[4] Sun Yat Sen Univ, Sch Publ Hlth, Guangzhou 510080, Peoples R China

[5] Ctr Dis Control & Prevent Guangdong Prov, Guangzhou 510300, Peoples R China

[6] Chinese Acad Sci, S China Inst Bot, Grad Sch, Guangzhou 510300, Peoples R China

[7] Univ Massachusetts, Sch Med, Program Mol Med, Worcester, MA USA

来源：

VACCINE | 2005年 / 23卷 / 24期

关键词：

SARS coronavirus (SARS-CoV); inactivated vaccine; immunogenicity; Balb/c mouse;

D O I：

10.1016/j.vaccine.2004.11.073

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.

引用

页码：3196 / 3201

页数：6

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