Preservation of plant cell ultrastructure during immunolocalization of virus particles

被引：10

作者：

Ghoshroy, S

Citovsky, V ^{[1
]}

机构：

[1] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA

[2] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 74卷 / 02期

关键词：

tobamoviruses; turnip vein clearing virus; coat protein; immunostaining; electron microscopy; plant tissue;

D O I：

10.1016/S0166-0934(98)00084-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Most immunoelectron microscopy techniques used for ultrastructural analyses of virus-infected plant tissues significantly compromise cellular membranous structures as well as overall contrast and resolution of the image. Here, we describe a protocol which avoids these flaws but retains full antigenicity of the sample. A direct comparison of the conventional and the improved electron immunostaining procedures is presented using tobacco and Arabidopsis thaliana plants infected with turnip vein clearing virus. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：223 / 229

页数：7

相关论文

共 15 条

[1] Phloem-specific expression of the tobacco mosaic virus movement protein alters carbon metabolism and partitioning in transgenic potato plants [J].

Almon, E ;

Horowitz, M ;

Wang, HL ;

Lucas, WJ ;

Zamski, E ;

Wolf, S .

PLANT PHYSIOLOGY, 1997, 115 (04) :1599-1607

[2] THE TOBACCO MOSAIC VIRUS-30K MOVEMENT PROTEIN IN TRANSGENIC TOBACCO PLANTS IS LOCALIZED TO PLASMODESMATA [J].

ATKINS, D ;

HULL, R ;

WELLS, B ;

ROBERTS, K ;

MOORE, P ;

BEACHY, RN .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :209-211

[3] SECONDARY PLASMODESMATA ARE SPECIFIC SITES OF LOCALIZATION OF THE TOBACCO MOSAIC-VIRUS MOVEMENT PROTEIN IN TRANSGENIC TOBACCO PLANTS [J].

DING, B ;

HAUDENSHIELD, JS ;

HULL, RJ ;

WOLF, S ;

BEACHY, RN ;

LUCAS, WJ .

PLANT CELL, 1992, 4 (08) :915-928

[4] LOCALIZATION BY ELECTRON MICROSCOPY OF HELA CELL SURFACE ENZYMES SPLITTING ADENOSINE TRIPHOSPHATE [J].

EPSTEIN, MA ;

HOLT, SJ .

JOURNAL OF CELL BIOLOGY, 1963, 19 (02) :325-+

[5] Inhibition of plant viral systemic infection by non-toxic concentrations of cadmium [J].

Ghoshroy, S ;

Freedman, K ;

Lartey, R ;

Citovsky, V .

PLANT JOURNAL, 1998, 13 (05) :591-602

[6] FLAT EMBEDDING AND IMMUNOLABELING OF SW-1116 COLON-CARCINOMA CELLS IN LR WHITE - AN IMPROVED TECHNIQUE IN LIGHT AND ELECTRON-MICROSCOPY [J].

GOPING, G ;

YEDGAR, S ;

POLLARD, HB ;

KUIJPERS, GAJ .

MICROSCOPY RESEARCH AND TECHNIQUE, 1992, 21 (01) :1-9

[7] Movement and subcellular localization of a tobamovirus in Arabidopsis [J].

Lartey, R ;

Ghoshroy, S ;

Ho, J ;

Citovsky, V .

PLANT JOURNAL, 1997, 12 (03) :537-545

[8] OCCURRENCE OF A VEIN-CLEARING TOBAMOVIRUS IN TURNIP [J].

LARTEY, RT ;

HARTSON, SD ;

PENNINGTON, RE ;

SHERWOOD, JL ;

MELCHER, U .

PLANT DISEASE, 1993, 77 (01) :21-24

[9] ARABIDOPSIS-THALIANA [J].

MEYEROWITZ, EM .

ANNUAL REVIEW OF GENETICS, 1987, 21 :93-111

[10] Viral coat protein is targeted to, but does not gate, plasmodesmata during cell-to-cell movement of potato virus X [J].

Oparka, KJ ;

Roberts, AG ;

Roberts, IM ;

Prior, DAM ;

SantaCruz, S .

PLANT JOURNAL, 1996, 10 (05) :805-813