Immobilization of anticoagulant-loaded liposomes on cell surfaces by DNA hybridization

被引：23

作者：

Chen, Hao ^{[1
]}

Teramura, Yuji ^{[2
]}

Iwata, Hiroo ^{[1
]}

机构：

[1] Kyoto Univ, Inst Frontier Med Sci, Dept Reparat Mat, Sakyo Ku, Kyoto 6068507, Japan

[2] Kyoto Univ, Radioisotope Res Ctr, Sakyo Ku, Kyoto 6068501, Japan

来源：

BIOMATERIALS | 2011年 / 32卷 / 31期

关键词：

Islet of Langerhans; Poly(ethylene glycol)-lipid; Argatroban; Liposome; polyDNA hybridization; Surface modification; CLINICAL ISLET TRANSPLANTATION; MEDIATED INFLAMMATORY REACTION; TISSUE FACTOR; LANGERHANS; BLOOD; THROMBOMODULIN; INHIBITION; SECRETION; UROKINASE; PREVENTS;

D O I：

10.1016/j.biomaterials.2011.07.002

中图分类号：

R318 [生物医学工程];

学科分类号：

100103 [病原生物学];

摘要：

An unresolved obstacle in transplantation of islets of Langerhans is the early graft loss caused by thrombotic reactions on the surface of islets after intraportal transplantation. We investigated a versatile method for modifying the surface of islets with liposomes carrying the anticoagulant argatroban using an amphiphilic poly(ethylene glycol)-phospholipid conjugate derivative (PEG-lipid) and DNA hybridization. Argatroban was gradually released from the liposomes on the islets, and antithrombic activity was detected in culture medium. Modified islets retained the ability to control insulin release in response to glucose concentration changes. Although we mainly examined surface modification of islets, this technique may be useful for immobilizing various types of small molecules on cells and tissues and thus may have many applications in cell therapy and regenerative medicine. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：7971 / 7977

页数：7

共 21 条

[1]

Incompatibility between human blood and isolated islets of Langerhans - A finding with implications for clinical intraportal islet transplantation? [J].

Bennet, W ;

Sundberg, B ;

Groth, CG ;

Brendel, MD ;

Brandhorst, D ;

Brandhorst, H ;

Bretzel, RG ;

Elgue, G ;

Larsson, R ;

Nilsson, B ;

Korsgren, O .

DIABETES, 1999, 48 (10) :1907-1914

[2]

Islet surface heparinization prevents the instant blood-mediated inflammatory reaction in islet transplantation [J].

Cabric, Sanja ;

Sanchez, Javier ;

Lundgren, Torbjorn ;

Foss, Aksel ;

Felldin, Marie ;

Kallen, Ragnar ;

Salmela, Kaija ;

Tibell, Annika ;

Tufveson, Gunnar ;

Larsson, Rolf ;

Korsgren, Olle ;

Nilsson, Bo .

DIABETES, 2007, 56 (08) :2008-2015

[3]

Co-immobilization of urokinase and thrombomodulin on islet surfaces by poly(ethylene glycol)-conjugated phospholipid [J].

Chen, Hao ;

Teramura, Yuji ;

Iwata, Hiroo .

JOURNAL OF CONTROLLED RELEASE, 2011, 150 (02) :229-234

[4]

Activated protein C preserves functional islet mass after intraportal transplantation -: A novel link between endothelial cell activation, thrombosis, inflammation, and islet cell death [J].

Contreras, JL ;

Eckstein, C ;

Smyth, CA ;

Bilbao, G ;

Vilatoba, M ;

Ringland, SE ;

Young, C ;

Thompson, JA ;

Fernández, JA ;

Griffin, JH ;

Eckhoff, DE .

DIABETES, 2004, 53 (11) :2804-2814

[5]

Vulnerability of islets in the immediate posttransplantation period - Dynamic changes in structure and function [J].

Davalli, AM ;

Scaglia, L ;

Zangen, DH ;

Hollister, J ;

BonnerWeir, S ;

Weir, GC .

DIABETES, 1996, 45 (09) :1161-1167

[6]

POTASSIUM IONS AND SECRETION OF INSULIN BY ISLETS OF LANGERHANS INCUBATED IN VITRO [J].

HOWELL, SL ;

TAYLOR, KW .

BIOCHEMICAL JOURNAL, 1968, 108 (01) :17-&

[7]

Low molecular weight dextran sulfate: A strong candidate drug to block IBMIR in clinical islet transplantation [J].

Johansson, H ;

Goto, M ;

Siegbahn, A ;

Elgue, G ;

Korsgren, O ;

Nilsson, B .

AMERICAN JOURNAL OF TRANSPLANTATION, 2006, 6 (02) :305-312

[8]

Tissue factor produced by the endocrine cells of the islets of Langerhans is associated with a negative outcome of clinical islet transplantation [J].

Johansson, H ;

Lukinius, A ;

Moberg, L ;

Lundgren, T ;

Berne, C ;

Foss, A ;

Felldin, M ;

Källen, R ;

Salmela, K ;

Tibe, A ;

Tufveson, G ;

Ekdahl, KN ;

Elgue, G ;

Korsgren, O ;

Nilsson, B .

DIABETES, 2005, 54 (06) :1755-1762

[9]

JOHNSON PC, COMMUNICATION

[10]

Production of tissue factor by pancreatic islet cells as a trigger of detrimental thrombotic reactions in clinical islet transplantation [J].

Moberg, L ;

Johansson, H ;

Lukinius, A ;

Berne, C ;

Foss, A ;

Källen, R ;

Ostraat, O ;

Salmela, K ;

Tibell, A ;

Tufveson, G ;

Elgue, G ;

Ekdahl, KN ;

Korsgren, O ;

Nilsson, B .

LANCET, 2002, 360 (9350) :2039-2045

← 1 2 3 →