Determination of tryptophan by high-performance liquid chromatography of alkaline hydrolysates with spectrophotometric detection

被引：198

作者：

Yust, MM ^{[1
]}

Pedroche, J ^{[1
]}

Girón-Calle, J ^{[1
]}

Vioque, J ^{[1
]}

Millán, F ^{[1
]}

Alaiz, M ^{[1
]}

机构：

[1] CSIC, Inst Grasa & Derivados, Seville 41012, Spain

来源：

FOOD CHEMISTRY | 2004年 / 85卷 / 02期

关键词：

tryptophan; feedstuff; sodium hydrolysis; high-performance liquid chromatography;

D O I：

10.1016/j.foodchem.2003.07.026

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A procedure for quantitation of tryptophan in feedstuff is described. It consist of hydrolysis in sodium hydroxide at 100degreesC for 4 h, neutralization of the resulting hydrolysate to pH 7, dilution with sodium borate buffer (pH 9), and analysis by reverse-phase high-performance liquid chromatography with spectrophotometric determination of tryptophan at 280 nm. The recovery of tryptophan from lysozyme, added to some feedstuff before hydrolysis, ranges from 98.6 to 100%. A reduction of the time of analysis has been achieved as compared to previous methods for analysis of tryptophan. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：317 / 320

页数：4

共 21 条

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[2]

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