Nasal immunization with subunit proteosome influenza vaccines induces serum HAI, mucosal IgA and protection against influenza challenge

被引：58

作者：

Plante, M ^{[1
]}

Jones, T ^{[1
]}

Allard, F ^{[1
]}

Torossian, K ^{[1
]}

Gauthier, J ^{[1
]}

St-Félix, N ^{[1
]}

White, GL ^{[1
]}

Lowell, GH ^{[1
]}

Burt, DS ^{[1
]}

机构：

[1] Intellivax Int Inc, St Laurent, PQ H4S 2A1, Canada

来源：

VACCINE | 2001年 / 20卷 / 1-2期

关键词：

nasal immunization; proteosomes; influenza; vaccine;

D O I：

10.1016/S0264-410X(01)00268-7

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The immunogenicity of a mucosally delivered subunit influenza vaccine was assessed in mice. Split influenza virus vaccine (sFlu) was formulated with proteosomes (Pr-sFlu), administered intranasally, and the induced immunity was compared with the responses elicited by sFlu alone given either intramuscularly or intranasally. Intranasal (i.n.) immunization with Pr-sFlu induced specific serum IgG and hemagglutination inhibition (HAI) titers comparable to or better than those induced by intramuscular (i.m.) sFlu, and in contrast to sFlu alone, i.n. Pr-sFlu also induced high levels of influenza-specific IgA in lung and nasal washes. Mice receiving i.n. Pr-sFlu were completely protected against live virus challenge, as were mice immunized by injection with sFlu alone. The i.n. Pr-sFlu elicited cytokine responses polarized towards a type 1 phenotype whereas those elicited by sFlu alone were of a mixed type 1/type 2 phenotype. The data strongly suggest that i.n. proteosome-formulated influenza antigens are highly effective and are excellent candidates for a non-invasive human vaccine. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：218 / 225

页数：8

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[1] PNEUMONIA AND INFLUENZA DEATHS DURING EPIDEMICS - IMPLICATIONS FOR PREVENTION [J].