Superoxide release and NADPH oxidase components in mature human phagocytes: Correlation between functional capacity and amount of functional proteins

被引:68
作者
Yagisawa, M
Yuo, A
Yonemaru, M
ImajohOhmi, S
Kanegasaki, S
Yazaki, Y
Takaku, F
机构
[1] INT MED CTR JAPAN, RES INST,DEPT HEMATOL,DIV HEMATOPOIET DISORDER, SHINJUKU KU, TOKYO 162, JAPAN
[2] TOKYO MED COLL, DEPT INTERNAL MED 1, TOKYO 160, JAPAN
[3] UNIV TOKYO, INST MED SCI, TOKYO 108, JAPAN
[4] UNIV TOKYO, FAC MED, DEPT INTERNAL MED 3, TOKYO 113, JAPAN
关键词
D O I
10.1006/bbrc.1996.1691
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We evaluated the interrelationship between the respiratory activity and amount of proteins responsible for this function in normal and subnormal human phagocytes, neutrophils, eosinophils, monocytes, and macrophages. The superoxide-producing capacity was eosinophils>neutrophils>monocytes=macrophages when the cells were stimulated with chemotactic peptide or phorbol ester. Consonant with this finding, the protein content of three essential components of phagocyte oxidase (p22-phox, p67-phox, and p47-phox) was also eosinophils>neutrophils>monocytes=macrophages. On the other hand, the amount of another essential component, gp91-phox, was macrophage>neutrophils>eosinophils>monocytes. These findings together indicate an overall positive interrelationship between protein content and its responsible function, though only gp91-phox was not associated with the functional capacity and low amounts of this component supported the increased respiratory bunt activity of eosinophils. (C) 1996 Academic Press, Inc.
引用
收藏
页码:510 / 516
页数:7
相关论文
共 21 条
  • [1] THE RESPIRATORY BURST OF PHAGOCYTES
    BABIOR, BM
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1984, 73 (03) : 599 - 601
  • [2] DECHATELET LR, 1977, BLOOD, V50, P525
  • [3] SUPEROXIDE PRODUCTION BY PHAGOCYTIC LEUKOCYTES
    DRATH, DB
    KARNOVSKY, ML
    [J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1975, 141 (01) : 257 - 262
  • [4] HOIDAL JR, 1981, AM REV RESPIR DIS, V123, P85
  • [5] IKI S, IN PRESS EXP HEMATOL
  • [6] INDUCTION OF ESSENTIAL COMPONENTS OF THE SUPEROXIDE GENERATING-SYSTEM IN HUMAN MONOBLASTIC LEUKEMIA U937 CELLS
    KIKUCHI, H
    FUJINAWA, T
    KURIBAYASHI, F
    NAKANISHI, A
    IMAJOHOHMI, S
    GOTO, M
    KANEGASAKI, S
    [J]. JOURNAL OF BIOCHEMISTRY, 1994, 116 (04) : 742 - 746
  • [7] LEVY R, 1990, J IMMUNOL, V145, P2595
  • [8] THE SUPEROXIDE-GENERATING OXIDASE OF PHAGOCYTIC-CELLS - PHYSIOLOGICAL, MOLECULAR AND PATHOLOGICAL ASPECTS
    MOREL, F
    DOUSSIERE, J
    VIGNAIS, PV
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1991, 201 (03): : 523 - 546
  • [9] RESPIRATORY BURST OF NORMAL HUMAN EOSINOPHILS
    PETRECCIA, DC
    NAUSEEF, WM
    CLARK, RA
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 1987, 41 (04) : 283 - 288
  • [10] THE GENETIC-BASIS OF CHRONIC GRANULOMATOUS-DISEASE
    ROOS, D
    [J]. IMMUNOLOGICAL REVIEWS, 1994, 138 : 121 - 157