Four Trypanosoma brucei fatty acyl-CoA synthetases:: fatty acid specificity of the recombinant proteins

被引：14

作者：

Jiang, DW ^{[1
]}

Englund, PT ^{[1
]}

机构：

[1] Johns Hopkins Med Sch, Dept Biol Chem, Baltimore, MD 21205 USA

来源：

BIOCHEMICAL JOURNAL | 2001年 / 358卷 / 03期

关键词：

ACS signature motif; enzyme-coupled assay; fatty acid chain length; glycosyl phosphatidylinositol myristoylation; recombinant acyl-CoA synthetases (ACS);

D O I：

10.1042/0264-6021:3580757

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As part of our investigation of fatty acid metabolism in Trypanosoma brucei, we have expressed four acyl-CoA synthetase (TbACS) genes in Esherichia coli. The recombinant proteins, with His-tags on their C-termini, were purified to near homogeneity using nickel-chelate affinity chromatography. Although these. enzymes are highly homologous, they have distinct specificities for fatty acid chain length. TbACS1 prefers saturated fatty acids in the range C-11:0 to C-14:0 and TbACS2 prefers shorter fatty acids, mainly C-10:0. TbACS3 and 4, which have 95% sequence identity, have similar specificities, favouring fatty acids between C-14:0 and C-17:0. In addition, TbACS1, 3 and 4 function well with a variety of unsaturated fatty acids.

引用

页码：757 / 761

页数：5

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