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Inhibition activity of sulfated polysaccharide of Sepiella maindroni ink on matrix metalloproteinase (MMP)-2

被引：55
作者：
Wang, Subo [2 ]
Cheng, Yanna [1 ]
Wang, Fengshan [1 ]
Sun, Lirui [1 ]
Liu, Chunhui [1 ]
Chen, Guanjun [2 ]
Li, Yuhua [1 ]
Ward, S. G. [3 ]
Qu, Xianjun [1 ]
机构：
[1] Shandong Univ, Sch Pharmaceut Sci, Jinan 250012, Peoples R China
[2] Shandong Univ, Sch Ocean Sci, Weihai 264209, Peoples R China
[3] Univ Bath, Dept Pharm & Pharmacol, Bath BA2 7AV, Avon, England
来源：
BIOMEDICINE & PHARMACOTHERAPY | 2008年 / 62卷 / 05期
基金：
中国国家自然科学基金;
关键词：
S. maindroni ink polysaccharide (SIP); sulfated polysaccharide (SIP-SII); matrix metalloproteinases; MMP-2; tumor invasion; migration;
D O I：
10.1016/j.biopha.2008.01.018
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 [基础医学];
摘要：
SIP-SII is the sulfated S. maindroni ink polysaccharide (SIP) isolated from cuttlefish Sepiella maindroni. SIP-SII weakly inhibited tumor cell growth without cytotoxicity in vitro assay. Herein, we examined the effects of SIP-SII on the expression of matrix metalloproteinase MMP-2 and MMP-9 as well as tumor cell invasion and migration. SIP-SII (0.8-500 mu g/ml) significantly decreased the expression of MMP-2 activity in human ovarian carcinoma cells SKOV3 as evidenced by the gelatin zymography analysis. No significant decrease of MMP-9 was detected in the cell line after SIP-SII treatment. The expression of MMP-2 was also evaluated using Western blot analysis. The results showed that SIP-SII inhibited the expression of MMP-2 in SKOV3 and human umbilical vein vascular endothelial cells ECV304 after 24 h incubation. Furthermore, the activity of invasion and migration of SKOV3 and ECV304 cells were measured. SIP-SII displayed an inhibitory effect on the penetration of SKOV3 cells through Matrigel-coated membrane in transwell chamber. A significant inhibition of ECV304 cell migration was observed in the presence of SIP-SIT. These results suggest that SIP-SII might suppress invasion and migration of carcinoma cells via inhibition of MMP-2 proteolytic activity. (C) 2008 Published by Elsevier Masson SAS.
引用
收藏
页码：297 / 302
页数：6
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