The crystal structure of flap endonuclease-1 from Methanococcus jannaschii

被引:145
作者
Hwang, KY [1 ]
Baek, K [1 ]
Kim, HY [1 ]
Cho, Y [1 ]
机构
[1] Korea Inst Sci & Technol, Struct Biol Ctr, Seoul 130650, South Korea
关键词
D O I
10.1038/1406
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Angstrom resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that the loop is critical for recognition and cleavage of the junction between single and double-stranded regions of flap DNA.
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页码:707 / 713
页数:7
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