Molecular cloning and identification of UTR1 of a yeast Saccharomyces cerevisiae as a gene encoding an NAD kinase

被引：56

作者：

Kawai, S ^{[1
]}

Suzuki, S ^{[1
]}

Mori, S ^{[1
]}

Murata, K ^{[1
]}

机构：

[1] Kyoto Univ, Food Sci Res Inst, Kyoto 6110011, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 200卷 / 02期

关键词：

NAD kinase; Saccharomyces cerevisiae; UTR1; ferrireductase system; NAD; NADP;

D O I：

10.1016/S0378-1097(01)00215-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

UTR1 of the yeast Saccharomyces cerevisiae was cloned from the genomic DNA by polymerase chain reaction and expressed in Escherichia coli. Characterization of the purified UTR1p revealed that UTR1p is a NAD kinase consisting of six identical subunits with a molecular mass of 60 kDa. UTR1p specifically phosphorylated NAD in the presence of ATP, dATP, or CTP as phosphoryl donors, and was most active at pH 8.0, 30 degreesC. K-m values of UTR1p for NAD and ATP were determined to be 0.50 mM and 0.60 mM, respectively. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：181 / 184

页数：4

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