Effects of malaria infection on vitellogenesis in Anopheles gambiae during two gonotrophic cycles

被引:58
作者
Ahmed, AM
Maingon, R
Romans, P
Hurd, H
机构
[1] Univ Keele, Sch Life Sci, Ctr Appl Entomol & Parasitol, Keele ST5 5BG, Staffs, England
[2] Univ Toronto, Dept Zool, Toronto, ON M5S 3G5, Canada
关键词
Anopheles gambiae; vitellogenin mRNA; fecundity reduction; vitellogenesis; Plasmodium yoelli nigeriensis;
D O I
10.1046/j.0962-1075.2001.00273.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We report changes in the abundance of vitellogenin (Vg) mRNA, and concentration of haemolymph Vg and ovarian vitellin (Vn) in Anopheles gambiae following infection with Plasmodium yoelli nigeriensis. A parasite-induced reduction in Vg mRNA abundance was first detected 24 h after feeding on an infective blood meal, when ookinetes were invading the midgut. During a second gonotrophic cycle postinfection, developing oocysts reduced Vg mRNA abundance by up to 33% and the effect was detected from 2 h post blood meal. Concentrations of Vg were initially reduced by infection during the second cycle, as predicted from Vg mRNA measurements. However, after 24 h, excess Vg had accumulated in the haemolymph. This accumulation may be due to impaired uptake, since ovarian vitellin accumulation was significantly decreased by infection during both gonotrophic cycles.
引用
收藏
页码:347 / 356
页数:10
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