Ribosomal RNA sequencing reveals differences between the genotypes of Giardia isolates recovered from humans and dogs living in the same locality

被引:352
作者
Hopkins, RM [1 ]
Meloni, BP [1 ]
Groth, DM [1 ]
Wetherall, JD [1 ]
Reynoldson, JA [1 ]
Thompson, RCA [1 ]
机构
[1] MURDOCH UNIV,SCH VET STUDIES,STATE AGR BIOTECHNOL CTR,MURDOCH,WA 6150,AUSTRALIA
关键词
D O I
10.2307/3284315
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A polymerase chain reaction-based method for genotyping Giardia duodenalis isolates using a polymorphic region near the 5' end of the small subunit ribosomal (SSU) RNA gene is described. Analysis was performed using Giardia cysts purified directly from feces. isolates were collected from humans and dogs living in isolated Aboriginal communities where Ginrrlin infections are highly endemic. This is the first report of the genetic characterization of Giardia from dogs and humans living in the same locality. Comparison of the SSU-rRNA sequences from 13 human and 9 dog isolates revealed 4 different genetic groups. Groups 1 and 2 contained all of the human isolates. whereas groups 3 and 4 consisted entirely of Giardia samples recovered from dogs. One dog sample contained templates from both groups 2 and 3. These results suggest that zoonotic transmission of Giardia infections between humans and dogs does not occur frequently in these communities. The dog-associated SSU-rRNA sequences have not been reported before, suggesting a new G. duodenalis subgroup. A genetic basis for the differences observed between the groups was supported by sequence analysis of 9 in vitro cultured isolates that were placed into the same genetic groups established by enzyme electrophoresis.
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页码:44 / 51
页数:8
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