Amplified detection of DNA and analysis of single-base mismatches by the catalyzed deposition of gold on Au-nanoparticles

被引：137

作者：

Weizmann, Y ^{[1
]}

Patolsky, F ^{[1
]}

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

ANALYST | 2001年 / 126卷 / 09期

关键词：

D O I：

10.1039/b106613g

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel amplification route for DNA detection based on the deposition of gold on a 10 nm Au-colloid/avidin conjugate label acting as a 'seeding' catalyst, is described. Microgravimetric quartz-crystal-microbalance measurements are employed to transduce the catalyzed deposition of gold on the piezoelectric crystals. Three different DNA detection schemes are described: (i) analysis of a 27-base nucleic acid fragment; (ii) analysis of the entire M13 phi DNA (7229 bases); and (iii) detection of a single-base mismatch in a DNA. Ultrasensitive detection of DNA is accomplished by the catalyzed deposition of gold, detection limit similar to 1 x 10(-15) M.

引用

页码：1502 / 1504

页数：3

共 15 条

[1] Liposomes labeled with biotin and horseradish peroxidase: A probe for the enhanced amplification of antigen-antibody or oligonucleotide-DNA sensing processes by the precipitation of an insoluble product on electrodes
Alfonta, L
Singh, AK
Willner, I
[J]. ANALYTICAL CHEMISTRY, 2001, 73 (01) : 91 - 102
[2] Amplified microgravimetric quartz crystal-microbalance analyses of oligonucleotide complexes: a route to a Tay-Sachs biosensor device
Bardea, A
Dagan, A
Ben-Dov, I
Amit, B
Willner, I
[J]. CHEMICAL COMMUNICATIONS, 1998, (07) : 839 - 840
[3] Hydroxylamine seeding of colloidal Au nanoparticles in solution and on surfaces
Brown, KR
Natan, MJ
[J]. LANGMUIR, 1998, 14 (04) : 726 - 728
[4] Single-base mismatch detection based on charge transduction through DNA
Kelley, SO
Boon, EM
Barton, JK
Jackson, NM
Hill, MG
[J]. NUCLEIC ACIDS RESEARCH, 1999, 27 (24) : 4830 - 4837
[5] Mikkelsen Susan R., 1996, Electroanalysis, V8, P15, DOI 10.1002/elan.1140080104
[6] SEQUENCE-SELECTIVE BIOSENSOR FOR DNA-BASED ON ELECTROACTIVE HYBRIDIZATION INDICATORS
MILLAN, KM
MIKKELSEN, SR
[J]. ANALYTICAL CHEMISTRY, 1993, 65 (17) : 2317 - 2323
[7] Patolsky F, 2000, ANGEW CHEM INT EDIT, V39, P940, DOI 10.1002/(SICI)1521-3773(20000303)39:5<940::AID-ANIE940>3.3.CO
[8] 2-P
[9] Dendritic amplification of DNA analysis by oligonucleotide-functionalized Au-nanoparticles
Patolsky, F
Ranjit, KT
Lichtenstein, A
Willner, I
[J]. CHEMICAL COMMUNICATIONS, 2000, (12) : 1025 - 1026
[10] Enzyme-linked amplified electrochemical sensing of oligonucleotide-DNA interactions by means of the precipitation of an insoluble product and using impedance spectroscopy
Patolsky, F
Katz, E
Bardea, A
Willner, I
[J]. LANGMUIR, 1999, 15 (11) : 3703 - 3706

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