In vitro response of Actinidia deliciosa explants to different BA incubation periods

被引：28

作者：

Moncaleán, P

Rodríguez, A

Fernández, B

机构：

[1] Univ Oviedo, Dept Biol Organismos & Sistemas, Unidad Fisiol Vegetal, E-33071 Oviedo, Spain

[2] Inst Univ Biotechnol Asturias, E-33071 Oviedo, Spain

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2001年 / 67卷 / 03期

关键词：

acclimatization; incubation periods; liquid medium; micropropagation; rooting;

D O I：

10.1023/A:1012732429147

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Actinidia deliciosa apical shoots were cultured in MS liquid medium with cellulose plugs as support for the explants. Different BA (4.4 muM) incubation periods were tested in order to improve the effectiveness of the micropropagation system by reducing the cytokinin incubation period. At the end of 3 successive subcultures, the explants were analysed and a number of parameters (number, weight and length of shoots, presence and weight of callus, multiplication index, etc.) were measured. Different BA incubation periods have a long-term effect since the best results at the end of multiplication stage were not followed by better growth at the end of the acclimatised period studied. The highest quality plants were those obtained from culturing in the presence of BA for 1 day. Our results show that BA not only has an important effect on the different phases of micropropagation, but will also regulate the future development of the regenerants.

引用

页码：257 / 266

页数：10

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