A novel polyurethane/cellulose fibrous scaffold for cardiac tissue engineering

被引:63
作者
Chen, Po-Hsuen [1 ]
Liao, Hsueh-Chung [2 ]
Hsu, Sheng-Hao [3 ]
Chen, Rung-Shu [4 ]
Wu, Ming-Chung [5 ]
Yang, Yi-Fan [6 ]
Wu, Chau-Chung [7 ]
Chen, Min-Huey [4 ]
Su, Wei-Fang [2 ]
机构
[1] Natl Taiwan Univ, Sch Dent, Inst Oral Biol, Taipei 10002, Taiwan
[2] Natl Taiwan Univ, Dept Mat Sci & Engn, Taipei 10617, Taiwan
[3] Natl Taiwan Univ, Inst Polymer Sci & Engn, Taipei 10617, Taiwan
[4] Natl Taiwan Univ, Sch Dent, Grad Inst Clin Dent, Taipei 10002, Taiwan
[5] Chang Gung Univ, Dept Chem & Mat Engn, Taoyuan 33302, Taiwan
[6] Natl Taiwan Univ Hosp, Dept Internal Med, Taipei 10002, Taiwan
[7] Natl Taiwan Univ, Coll Med, Dept Primary Care Med, Taipei 10002, Taiwan
关键词
CELL-DERIVED CARDIOMYOCYTES; IN-VITRO; MYOCARDIAL REGENERATION; FIBER DIAMETER; SMOOTH-MUSCLE; HEART-TISSUE; ELECTROSPUN; NANOFIBERS; REPAIR; FABRICATION;
D O I
10.1039/c4ra12486c
中图分类号
O6 [化学];
学科分类号
070301 [无机化学];
摘要
The present work demonstrates a biomimetic electrospun scaffold based on polyurethane (PU) and ethyl cellulose (EC), featuring uniform fibrous nanostructures and three dimensional porous networks. The relationship between processing conditions and fibrous nanostructures is established which guides the rational processing with tunable fiber diameters. Additionally, the developed scaffold template reveals biocompatibility in retention and proliferation of cardiac myoblast H9C2 cells. The high mechanical strength of the PU/EC scaffolds enables the processing and handling of an ultrathin patch. Their elastomeric characteristics revealed the compatibility between the patch and contractile tissues. Furthermore, anisotropic PU/EC scaffolds with aligned nanofibers were successfully fabricated, exhibiting higher mechanical strength and essential characteristics for the survival and function of cardiac cells with native anisotropy. This work demonstrates a bioengineered PU/EC fibrous scaffold with uniform nanostructural webs and provides insight into the relationships between processing control, nanostructures and associated properties, with promising potential in cardiac tissue engineering.
引用
收藏
页码:6932 / 6939
页数:8
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