Activity profiles of 309 ToxCast™ chemicals evaluated across 292 biochemical targets

被引：101

作者：

Knudsen, Thomas B. ^{[1
]}

Houck, Keith A. ^{[1
]}

Sipes, Nisha S. ^{[1
]}

Singh, Amar V. ^{[2
]}

Judson, Richard S. ^{[1
]}

Martin, Matthew T. ^{[1
]}

Weissman, Arthur ^{[3
]}

Kleinstreuer, Nicole C. ^{[1
]}

Mortensen, Holly M. ^{[1
]}

Reif, David M. ^{[1
]}

Rabinowitz, James R. ^{[1
]}

Setzer, R. Woodrow ^{[1
]}

Richard, Ann M. ^{[1
]}

Dix, David J. ^{[1
]}

Kavlock, Roberti. ^{[1
]}

机构：

[1] US EPA, Natl Ctr Computat Toxicol B205 01, Off Res & Dev, Res Triangle Pk, NC 27711 USA

[2] Lockheed Martin, Res Triangle Pk, NC USA

[3] Caliper Discovery Alliances & Serv, Hanover, MD USA

来源：

TOXICOLOGY | 2011年 / 282卷 / 1-2期

关键词：

High-throughput screening; Computational toxicology; ToxCast; Environmental chemicals; LIGAND-BINDING DOMAIN; ANDROGEN RECEPTOR; IN-VITRO; REPRODUCTIVE TOXICITY; ANTRAL FOLLICLES; KINASE; ACTIVATION; PESTICIDES; INHIBITOR; MECHANISM;

D O I：

10.1016/j.tox.2010.12.010

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Understanding the potential health risks posed by environmental chemicals is a significant challenge elevated by the large number of diverse chemicals with generally uncharacterized exposures, mechanisms, and toxicities. The present study is a performance evaluation and critical analysis of assay results for an array of 292 high-throughput cell-free assays aimed at preliminary toxicity evaluation of 320 environmental chemicals in EPA's ToxCast (TM) project (Phase I). The chemicals (309 unique, 11 replicates) were mainly precursors or the active agent of commercial pesticides, for which a wealth of in vivo toxicity data is available. Biochemical HTS (high-throughput screening) profiled cell and tissue extracts using semi-automated biochemical and pharmacological methodologies to evaluate a subset of G-protein coupled receptors (GPCRs), CYP450 enzymes (CYPs), kinases, phosphatases, proteases, HDACs, nuclear receptors, ion channels, and transporters. The primary screen tested all chemicals at a relatively high concentration 25 mu M concentration (or 10 mu M for CYP assays), and a secondary screen re-tested 9132 chemical-assay pairs in 8-point concentration series from 0.023 to 50 mu M (or 0.009-20 mu M for CYPs). Mapping relationships across 93,440 chemical-assay pairs based on half-maximal activity concentration (AC50) revealed both known and novel targets in signaling and metabolic pathways. The primary dataset, summary data and details on quality control checks are available for download at http://www.epa.gov/ncct/toxcast/. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：1 / 15

页数：15

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