Plasmid maintenance and physiology of a genetically engineered Escherichia coli strain during continuous L-carnitine production

被引：7

作者：

Bernal, Vicente ^{[1
]}

Gonzalez-Veracruz, Maria ^{[1
]}

Canovas, Manuel ^{[1
]}

Iborra, Jose L. ^{[1
]}

机构：

[1] Univ Murcia, Fac Quim, Dept Bioquim & Biol Mol B & Immunol, E-30100 Murcia, Spain

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 10期

关键词：

Escherichia coli; flow cytometry; genetic stability; L-carnitine; metabolic burden;

D O I：

10.1007/s10529-007-9432-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of immobilization on cell physiology and how this determines cell metabolic performance is an important concern for developing bioprocess. This is particularly true for genetically modified microorganisms and their genetic stability. For this reason the stability and physiological state of plasmid-bearing E. coli cells were ascertained by flow cytometry. Differences in the cellular DNA and protein content (15-20%) permit discrimination of control and plasmid-bearing cells, as well as adaptation to continuous cultivation conditions in both freely suspended and immobilized states to be monitored. Moreover, the observed metabolic burden due to maintenance and over-expression of plasmid-coded genetic material and slow cell growth in poorly-viable immobilized cells were found to be the main factors contributing to strain stabilization.

引用

页码：1549 / 1556

页数：8

共 28 条

[1] ANALYSIS OF CELL-SIZE AND DNA CONTENT IN EXPONENTIALLY GROWING AND STATIONARY-PHASE BATCH CULTURES OF ESCHERICHIA-COLI [J].