Characterization of Perina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene

被引：22

作者：

Chou, CM

Huang, CJ

Lo, CF

Kou, GH

Wang, CH

机构：

[1] NATL TAIWAN UNIV,DEPT PLANT PATHOL & ENTOMOL,TAIPEI 107,TAIWAN

[2] NATL TAIWAN UNIV,DEPT ZOOL,TAIPEI 107,TAIWAN

[3] ACAD SINICA,INST BIOL CHEM,TAIPEI,TAIWAN

来源：

JOURNAL OF INVERTEBRATE PATHOLOGY | 1996年 / 67卷 / 03期

关键词：

Polyhedrin gene; Perina nuda nucleopolyhedrovirus; baculovirus;

D O I：

10.1006/jipa.1996.0041

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

The Per ina nuda nucleopolyhedrovirus (PenuNPV) polyhedrin gene was located in EcoRI-G (6.3 kilobase pairs; kbp) and PstI-G (4.3 kbp) fragments of its genomic DNA. A portion of 1333 nucleotides (nt) containing this gene was sequenced. An open reading frame of 735 nt encoded a 245-amino-acid-long polyhedrin. A conserved TAAG motif which is associated with transcriptional start sites was identified 51 nt upstream of the translation initiation codon of PenuNPV polyhedrin gene. A putative polyadenylation signal, AATAAA, was found 116 nt downstream of the termination codon (TAA). Comparison of the amino acid sequences of PenuNPV polyhedrin with those of other NPVs showed that PenuNPV poyhedrin was most closely related to Orgyia pseudotsugata multiple NPV (OpMNPV) polyhedrin. (C) 1996 Academic Press, Inc.

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页码：259 / 266

页数：8

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