A simple, rapid method for HPLC analysis of lycopene isomers

被引：73

作者：

Ishida, BK ^{[1
]}

Ma, J ^{[1
]}

Chan, B ^{[1
]}

机构：

[1] ARS, Western Reg Res Ctr, USDA, Albany, CA 94710 USA

来源：

PHYTOCHEMICAL ANALYSIS | 2001年 / 12卷 / 03期

关键词：

HPLC analysis; lycopene; lycopene isomers; tomato;

D O I：

10.1002/pca.576

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid method for the extraction, separation and quantification of the geometric isomers of lycopene and beta -carotene from tomato fruit is described. Carotenoids in tomato were separated and eluted using a reversed-phase HPLC with a C-30 column and a mobile phase consisting of methyl-t-butyl ether, methanol and ethyl acetate, The system provided sharp resolution of cis- and trans-isomers of lycopene within approximately 23 min in contrast to the longer and more complex gradient procedures required by previously described methods. Experiments indicate that the stability of extracts of fresh tomato may be improved if stored at -20 degreesC, and that the presence of the antioxidant BHA has no apparent effect on stability. Copyright (C) 2001 John Wiley & Sons, Ltd.

引用

页码：194 / 198

页数：5

共 22 条

[1] Lycopene: Chemistry, biology, and implications for human health and disease [J].