Fluorimetric-enzymatic determination of glucose based on labelled glucose oxidase

被引：29

作者：

Sierra, JF ^{[1
]}

Galban, J ^{[1
]}

de Marcos, S ^{[1
]}

Castillo, JR ^{[1
]}

机构：

[1] Univ Zaragoza, Fac Sci, Dept Analyt Chem, GEAS,Analyt Spectrometry & Sensors Grp, E-50009 Zaragoza, Spain

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 368卷 / 1-2期

关键词：

fluorescence; glucose; labelled glucose oxidase; 7-hydroxycoumarin-4-acetic acid;

D O I：

10.1016/S0003-2670(98)00197-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The main problem of enzymatic determinations based on the intrinsic fluorescence of enzymes is the wavelength range at which these compounds are excited (usually in the UV region). This study presents a new alternative which partially avoids this problem. It is based on a prior coupling reaction of the enzyme to a fluorescent reagent which has optical properties in the visible region. The method is applied to the enzymatic determination of glucose, with glucose oxidase (GOx) coupled to 7-hydroxycoumarin-4-acetic acid as the fluorescent reagent. The labelling procedure as well as the reaction conditions are studied and the origin of the analytical signal is discussed. The method gives a linear response to glucose concentration from 5.0 x 10(-4) to 6.0 x 10(-3) M and is applied to glucose determination in fruit juices with good results. (C) 1998 Elsevier Science B.V.

引用

页码：97 / 104

页数：8

共 9 条

[1] DIRECT ELECTRICAL COMMUNICATION BETWEEN CHEMICALLY MODIFIED ENZYMES AND METAL-ELECTRODES .2. METHODS FOR BONDING ELECTRON-TRANSFER RELAYS TO GLUCOSE-OXIDASE AND D-AMINO-ACID OXIDASE [J].