Conjugation of 5-azido-3-oxapentyl glycosides with thiolated proteins through the use of thiophilic derivatives

被引：8

作者：

Fernández-Santana, V ^{[1
]}

González-Lio, R ^{[1
]}

Sarracent-Pérez, J ^{[1
]}

Verez-Bencomo, V ^{[1
]}

机构：

[1] Univ La Habana, Fac Quim, Lab Synthet Antigens, La Habana 10400, Cuba

来源：

GLYCOCONJUGATE JOURNAL | 1998年 / 15卷 / 06期

关键词：

spacer-arm glycosides; neoglycoprotein; conjugation; Lewis human blood group;

D O I：

10.1023/A:1006903524007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

5-azido-3-oxa-pentyl beta-D-galactopyranoside was prepared from diethylene glycol monochlorohydrin and used as a model of oligosaccharide hapten. After deprotection, a series of amides bearing thiophilic groups had been obtained through the terminal amino function and essayed in coupling reactions with thiolated BSA. Also several Lewis human blood group oligosaccharides had been conjugated with thiolated BSA demonstrating the usefulness of the methodology.

引用

页码：549 / 553

页数：5

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