The renin-angiotensin system in hybrid NG108-15 cells - Renin gene is from mouse neuroblastoma, angiotensinogen and angiotensin-converting enzyme genes are of rat glioma origin

被引：5

作者：

Laflamme, L

Brechler, V

Reudelhuber, TL

Gallo-Payet, N ^{[1
]}

Deschepper, CF

机构：

[1] Univ Sherbrooke, Fac Med, Serv Endocrinol, Sherbrooke, PQ J1H 5N4, Canada

[2] Clin Res Inst Montreal, Labs Mol Biochem Hypertens, Montreal, PQ H2W 1R7, Canada

[3] Clin Res Inst Montreal, Lab Neurobiol & Vasoact Peptides, Montreal, PQ H2W 1R7, Canada

来源：

REGULATORY PEPTIDES | 1998年 / 77卷 / 1-3期

基金：

英国医学研究理事会;

关键词：

renin-angiotensin system; angiotensin receptors; NG108-15; cells;

D O I：

10.1016/S0167-0115(98)00025-1

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Angiotensin II (Ang II) increases the level of tyrosine phosphorylation of several proteins in nondifferentiated NG108-15 cells, a hybrid derived from the fusion of mouse neuroblastoma and rat glioma cells. Conversely, incubation of NG108-15 cells with an angiotensin-converting enzyme (ACE) inhibitor decreased the basal level of tyrosine phosphorylation of proteins, suggesting that locally secreted Ang II may act as an autocrine regulator. By RT-PCR, we found that nondifferentiated NG108-15 cells contained the mRNA transcript of the rat angiotensinogen, mouse renin and rat ACE genes, thus confirming that NG108-15 cells contain all the elements of a local renin-angiotensin system. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：9 / 15

页数：7

共 41 条

[1] DISTINCT MODE OF MICROTUBULE-ASSOCIATED PROTEIN-2 EXPRESSION IN THE NEUROBLASTOMA-GLIOMA CELL-LINE 108CC15/NG108-15 [J].