Single- and double-strand breaks induced in plasmid DNA irradiated by ultra-soft X-ray

被引：9

作者：

Fayard, B

Touati, A

Sage, E

Abel, F

Champion, C

Chetoui, A

机构：

[1] Univ Paris 06, Phys Solides Grp, CNRS, UMR 75 88, F-75251 Paris 05, France

[2] Univ Paris 07, Phys Solides Grp, CNRS, UMR 75 88, F-75251 Paris, France

[3] Inst Curie, Sect Rech, CEA, CNRS,UMR 218, F-75248 Paris 05, France

来源：

JOURNAL DE CHIMIE PHYSIQUE ET DE PHYSICO-CHIMIE BIOLOGIQUE | 1999年 / 96卷 / 01期

关键词：

ultra-soft X-rays; carbon K-ionization; double-strand breaks;

D O I：

10.1051/jcp:1999123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to investigate the molecular consequences of a carbon K photo-ionization located on DNA, dry pBS plasmid samples were irradiated with ultra-soft X-rays at energies below and above the carbon K-threshold (E-K = 278 eV). Single- and double-strand breaks (ssb and dsb) were quantified after resolution of the three plasmid forms (supercoiled, relaxed circular, linear) by gel electrophoresis. A factor of 1.2 was found between the doses required at 250 eV and 380 eV to induce the same number of dsb per plasmid.

引用

页码：147 / 151

页数：5

共 6 条

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