Rigidity of circulating lymphocytes is primarily conferred by vimentin intermediate filaments

被引:124
作者
Brown, MJ
Hallam, JA
Colucci-Guyon, E
Shaw, S
机构
[1] NCI, Expt Immunol Branch, Human Immunol Sect, NIH, Bethesda, MD 20892 USA
[2] Inst Pasteur, Unite Biol Dev, Unite Rech Associee, CNRS, Paris, France
关键词
D O I
10.4049/jimmunol.166.11.6640
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Lymphocytes need rigidity while in circulation, but must abruptly become deformable to undergo transmigration into tissue. Previously, the control of leukocyte deformability has been attributed to microfilaments or microtubules, but the present studies demonstrate the greater importance of vimentin intermediate filaments (IFs). In circulating T lymphocytes, IFs form a distinctive spherical cage that undergoes a rapid condensation into a juxtanuclear aggregate during chemokine-induced polarization. Measurements of the resistance of peripheral blood T lymphocytes to global deformation demonstrate that their rigidity is primarily dependent on intact vimentin filaments. Microtubules, in contrast, are not sufficient to maintain rigidity. Thus, vimentin IFs are a primary source of structural support in circulating human lymphocytes, and their regulated collapse is likely to be an essential element in chemokine-induced transendothelial migration.
引用
收藏
页码:6640 / 6646
页数:7
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