Phosphorylation of amyloid-β at the serine 26 residue by human cdc2 kinase

被引：53

作者：

Milton, NGN

机构：

[1] Dept Mol Pathol, Wembley HA9 7YN, Middx, England

[2] Metagen Ltd, Wembley HA9 7YN, Middx, England

来源：

NEUROREPORT | 2001年 / 12卷 / 17期

关键词：

amyloid-beta; antisense peptide; cdc2; cyclin; olomoucine; phosphorylation;

D O I：

10.1097/00001756-200112040-00047

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The amyloid-beta (A beta) peptide has been implicated in the pathology of Alzheimer's disease (AD). Using an antisense peptide approach a novel interaction between A beta and the human cdc2 kinase was identified. The A beta 1-42, 1-40 and 25-35 peptides were shown to be substrates for the cdc2 kinase and phosphorylated on the Serine 26 residue. Phosphorylated A beta (pSA beta) was found in extracts from NT-2 neurons and AD brain. In NT-2 neurons the levels of pSA beta were increased in the presence of exogenous A beta and this increase was prevented by a cdc2 protein kinase inhibitor, olomoucine, that also prevented A beta cytotoxicity. The results from this study suggest that A beta phosphorylation by cdc2 could play a role in the brain pathology of AD. NeuroReport 12:3839-3844 (C) 2001 Lippincott Williams & Wilkins.

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页码：3839 / 3844

页数：6

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