Sodium bisulfite analysis of the methylation status of DNA from small portions of paraffin slides

被引：5

作者：

Anderson, AE ^{[1
]}

Haines, TR ^{[1
]}

Robinson, DP ^{[1
]}

Butcher, DT ^{[1
]}

Sadikovic, B ^{[1
]}

Rodenhiser, DI ^{[1
]}

机构：

[1] Univ Western Ontario, London, ON, Canada

来源：

BIOTECHNIQUES | 2001年 / 31卷 / 05期

关键词：

D O I：

10.2144/01315bm05

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

[No abstract available]

引用

页码：1004 / +

页数：3

共 10 条

[1] Promoter methylation analysis on micro-dissected paraffin-embedded tissues using bisulfite treatment and PCR-SSCP [J].

Bian, YS ;

Yan, P ;

Osterheld, MC ;

Fontolliet, C ;

Benhattar, J .

BIOTECHNIQUES, 2001, 30 (01) :66-+

[2]

CLARK SJ, 1994, NUCLEIC ACIDS RES, V22, P2990, DOI 10.1093/nar/22.15.2990

[3] METHYLATION ANALYSIS ON INDIVIDUAL CHROMOSOMES - IMPROVED PROTOCOL FOR BISULFITE GENOMIC SEQUENCING [J].

FEIL, R ;

CHARLTON, J ;

BIRD, AP ;

WALTER, J ;

REIK, W .

NUCLEIC ACIDS RESEARCH, 1994, 22 (04) :695-696

[4] A GENOMIC SEQUENCING PROTOCOL THAT YIELDS A POSITIVE DISPLAY OF 5-METHYLCYTOSINE RESIDUES IN INDIVIDUAL DNA STRANDS [J].

FROMMER, M ;

MCDONALD, LE ;

MILLAR, DS ;

COLLIS, CM ;

WATT, F ;

GRIGG, GW ;

MOLLOY, PL ;

PAUL, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (05) :1827-1831

[5] Identification of methylated cytosine from archival formalin-fixed paraffin-embedded specimens [J].

Kitazawa, S ;

Kitazawa, R ;

Maeda, S .

LABORATORY INVESTIGATION, 2000, 80 (02) :275-276

[6] CPG methylation within the 5′ regulatory region of the BRCA1 gene is tumor specific and includes a putative CREB binding site [J].

Mancini, DN ;

Rodenhiser, DI ;

Ainsworth, PJ ;

O'Malley, FP ;

Singh, SM ;

Xing, WR ;

Archer, TK .

ONCOGENE, 1998, 16 (09) :1161-1169

[7] A modified and improved method for bisulphite based cytosine methylation analysis [J].

Olek, A ;

Oswald, J ;

Walter, J .

NUCLEIC ACIDS RESEARCH, 1996, 24 (24) :5064-5066

[8] DEAMINATION OF CYTOSINE DERIVATIVES BY BISULFITE - MECHANISM OF REACTION [J].

SHAPIRO, R ;

DIFATE, V ;

WELCHER, M .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1974, 96 (03) :906-912

[9]

SHAPIRO R, 1973, J BIOL CHEM, V248, P4060

[10] COMPARISON OF BISULFITE MODIFICATION OF 5-METHYLDEOXYCYTIDINE AND DEOXYCYTIDINE RESIDUES [J].

WANG, RYH ;

GEHRKE, CW ;

EHRLICH, M .

NUCLEIC ACIDS RESEARCH, 1980, 8 (20) :4777-4790

← 1 →