Platelet derived growth factor-BB induced calcium transients cultured human peritoneal mesothelial cells

被引:6
作者
Bird, SD
Hasan, Q
Davis, PF
Walker, RJ
机构
[1] Dunedin Sch Med, Wellcome Med Res Inst, Dunedin, New Zealand
[2] Univ Otago, Wellington Sch Med, Dept Med, Renal Unit, Dunedin, New Zealand
关键词
D O I
10.1097/00002480-199811000-00013
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The peritoneal membrane is used as an artificial dialysis organ for patients with end-stage renal failure during continuous ambulatory peritoneal dialysis (CAPD). Resident peritoneal mast cells may influence the course of the inflammatory process during acute infection or prolonged exposure to dialysate in a paracrine fashion, with the release of preformed mediators, including platelet derived growth factor (PDGF), which may activate human peritoneal mesothelial cells (HPMC) and other peritoneal cells. PDGF receptor interactions have multiple intracellular effects, typified by cell proliferation, protein synthesis, cytoskeletal organization, extracellular matrix turnover, and angiogenesis. PDGF may mediate HPMC inflammatory response by stimulating cytokine release. The presence of transcripts for both PDGF-B polypeptide and PDGF-beta receptor in cultured HPMC was confirmed using a reverse transcriptase-polymerase chain reaction. To investigate the activity of PDGF-beta receptors in HPMC, the authors examined intracellular calcium (Ca-i(2+)) mobilization iri HPMC in response to PDGF-BB (100 ng/ml), histamine (1.0 mmol/L), and 4-brA23187 (1.0 mu mol/L) using the calcium indicator, fura-2. HPMC calcium mobilization in response to PDGF was assessed in Krebs-Ringer saline, with and without added external calcium (Ca-ext(2+)). HPMC responded to PDGF with a transient rise in Ca-i(2+) (approximately 1.6-fold) that returned to an elevated resting value. In the absence of Ca-ext(2+), PDGF produced a Ca-i(2+) transient indicative of Ca2+ release from intracellular stores. These results suggest that HPMC have functional PDGF-beta receptors that may bind and respond to PDGF in both an autocrine and paracrine fashion.
引用
收藏
页码:835 / 840
页数:6
相关论文
共 22 条
  • [1] BIRD SD, 1994, IN VITRO CELL DEV-AN, V30A, P420
  • [2] Bird SD, 1996, PERITON DIALYSIS INT, V16, P599
  • [3] BIRD SD, IN PRESS PERIT DIAL
  • [4] CLAESSONWELSH L, 1994, J BIOL CHEM, V269, P32023
  • [5] DILIBERTO PA, 1992, J BIOL CHEM, V267, P11888
  • [6] STIMULATION OF DNA-SYNTHESIS IN CULTURED PRIMARY HUMAN MESOTHELIAL CELLS BY SPECIFIC GROWTH-FACTORS
    GABRIELSON, EW
    GERWIN, BI
    HARRIS, CC
    ROBERTS, AB
    SPORN, MB
    LECHNER, JF
    [J]. FASEB JOURNAL, 1988, 2 (11) : 2717 - 2721
  • [7] GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
  • [8] THE EFFECT OF NEOMYCIN ON PDGF-INDUCED MITOGENIC RESPONSE AND ACTIN ORGANIZATION IN CULTURED HUMAN FIBROBLASTS
    HEDBERG, KM
    BELL, PB
    [J]. EXPERIMENTAL CELL RESEARCH, 1995, 219 (01) : 266 - 275
  • [9] PLATELET-DERIVED GROWTH-FACTOR - ISOFORM-SPECIFIC SIGNALING VIA HETERODIMERIC OR HOMODIMERIC RECEPTOR COMPLEXES
    HELDIN, CH
    OSTMAN, A
    ERIKSSON, A
    SIEGBAHN, A
    CLAESSONWELSH, L
    WESTERMARK, B
    [J]. KIDNEY INTERNATIONAL, 1992, 41 (03) : 571 - 574
  • [10] HJELLE JT, 1989, PERITON DIALYSIS INT, V9, P341