A light-actuated nanovalve derived from a channel protein

被引：424

作者：

Koçer, A

Walko, M

Meijberg, W

Feringa, BL

机构：

[1] BiOMaDe Technol Fdn, NL-9747 AG Groningen, Netherlands

[2] Univ Groningen, Stratingh Inst, Dept Organ & Mol Inorgan Chem, NL-9747 AG Groningen, Netherlands

[3] Univ Groningen, Ctr Mat Sci, NL-9747 AG Groningen, Netherlands

来源：

SCIENCE | 2005年 / 309卷 / 5735期

关键词：

D O I：

10.1126/science.1114760

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Toward the realization of nanoscale device control, we report a molecular valve embedded in a membrane that can be opened by illumination with long-wavelength ultraviolet (366 nanometers) tight and then resealed by visible irradiation. The valve consists of a channel protein, the mechanosensitive channel of large conductance (MscL) from Escherichia coli, modified by attachment of synthetic compounds that undergo tight-induced charge separation to reversibly open and close a 3-nanometer pore. The system is compatible with a classical encapsulation system, the liposome, and external photochemical control over transport through the channel is achieved.

引用

收藏

页码：755 / 758

页数：4

相关论文

共 27 条

[1] Release of thioredoxin via the mechanosensitive channel MscL during osmotic downshock of Escherichia coli cells [J].

Ajouz, B ;

Berrier, C ;

Garrigues, A ;

Besnard, M ;

Ghazi, E .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (41) :26670-26674

[2] Photoswitchable molecular receptors [J].

Alfimov, MV ;

Fedorova, OA ;

Gromov, SP .

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY, 2003, 158 (2-3) :183-198

[3] Gain-of-function mutations reveal expanded intermediate states and a sequential action of two gates in MscL [J].

Anishkin, A ;

Chiang, CS ;

Sukharev, S .

JOURNAL OF GENERAL PHYSIOLOGY, 2005, 125 (02) :155-170

[4] Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel MscL [J].

Berrier, C ;

Garrigues, A ;

Richarme, G ;

Ghazi, A .

JOURNAL OF BACTERIOLOGY, 2000, 182 (01) :248-251

[5] Effect of substitution on the change of refractive index in dithienylethenes: An ellipsometric study [J].

Bertarelli, C ;

Bianco, A ;

D'Amore, F ;

Gallazzi, MC ;

Zerbi, G .

ADVANCED FUNCTIONAL MATERIALS, 2004, 14 (04) :357-363

[6] Single residue substitutions that change the gating properties of a mechanosensitive channel in Escherichia coli [J].

Blount, P ;

Sukharev, SI ;

Schroeder, MJ ;

Nagle, SK ;

Kung, C .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (21) :11652-11657

[7] Mutations in a bacterial mechanosensitive channel change the cellular response to osmotic stress [J].

Blount, P ;

Schroeder, MJ ;

Kung, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (51) :32150-32157

[8] Photolabile protecting groups and linkers [J].

Bochet, CG .

JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 2002, (02) :125-142

[9] Structure of the MscL homolog from Mycobacterium tuberculosis:: A gated mechanosensitive ion channel [J].

Chang, G ;

Spencer, RH ;

Lee, AT ;

Barclay, MT ;

Rees, DC .

SCIENCE, 1998, 282 (5397) :2220-2226

[10] Estimation of the pore size of the large-conductance mechanosensitive ion channel of Escherichia coli [J].

Cruickshank, CC ;

Minchin, RF ;

LeDain, AC ;

Martinac, B .

BIOPHYSICAL JOURNAL, 1997, 73 (04) :1925-1931