Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1

被引:280
作者
Galy, V
Gadal, O
Fromont-Racine, M
Romano, A
Jacquier, A
Nehrbass, U
机构
[1] CNRS, URA 2582, Unite Biol Cellulaire Noyau, F-25724 Paris 15, France
[2] Inst Pasteur, CNRS, URA 2171, Unite Genet Interact Macromol, F-25724 Paris, France
关键词
D O I
10.1016/S0092-8674(03)01026-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated by perinuclearly located Mlp1. Deletion of MLP1 impairs retention while having no effect on mRNA splicing. The Mlp1-dependent leakage of intron-containing RNAs is increased in presence of ts-prp18Delta, a splicing mutant. When overall pre-mRNA levels are increased by deletion of RRP6, a nuclear exosome component, MLP1 deletion augments leakage of only the intron-containing portion of mRNAs. Our data suggest, moreover, that Mlp1-dependent retention is mediated via the 5' splice site. Intriguingly, we found Mlp-proteins to be present only on sections of the NE adjacent to chromatin. We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs.
引用
收藏
页码:63 / 73
页数:11
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