共 33 条
Nuclear retention of unspliced mRNAs in yeast is mediated by perinuclear Mlp1
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作者:

Galy, V
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机构: CNRS, URA 2582, Unite Biol Cellulaire Noyau, F-25724 Paris 15, France

Gadal, O
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机构: CNRS, URA 2582, Unite Biol Cellulaire Noyau, F-25724 Paris 15, France

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Romano, A
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机构: CNRS, URA 2582, Unite Biol Cellulaire Noyau, F-25724 Paris 15, France

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Nehrbass, U
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机构: CNRS, URA 2582, Unite Biol Cellulaire Noyau, F-25724 Paris 15, France
机构:
[1] CNRS, URA 2582, Unite Biol Cellulaire Noyau, F-25724 Paris 15, France
[2] Inst Pasteur, CNRS, URA 2171, Unite Genet Interact Macromol, F-25724 Paris, France
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D O I:
10.1016/S0092-8674(03)01026-2
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The molecular mechanism underlying the retention of intron-containing mRNAs in the nucleus is not understood. Here, we show that retention of intron-containing mRNAs in yeast is mediated by perinuclearly located Mlp1. Deletion of MLP1 impairs retention while having no effect on mRNA splicing. The Mlp1-dependent leakage of intron-containing RNAs is increased in presence of ts-prp18Delta, a splicing mutant. When overall pre-mRNA levels are increased by deletion of RRP6, a nuclear exosome component, MLP1 deletion augments leakage of only the intron-containing portion of mRNAs. Our data suggest, moreover, that Mlp1-dependent retention is mediated via the 5' splice site. Intriguingly, we found Mlp-proteins to be present only on sections of the NE adjacent to chromatin. We propose that at this confined site the perinuclear Mlp1 implements a quality control step prior to export, physically retaining faulty pre-mRNAs.
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页码:63 / 73
页数:11
相关论文
共 33 条
[1]
Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioning
[J].
Andrulis, ED
;
Zappulla, DC
;
Ansari, A
;
Perrod, S
;
Laiosa, CV
;
Gartenberg, MR
;
Sternglanz, R
.
MOLECULAR AND CELLULAR BIOLOGY,
2002, 22 (23)
:8292-8301

Andrulis, ED
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

Zappulla, DC
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

Ansari, A
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

Perrod, S
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

Laiosa, CV
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

Gartenberg, MR
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA

Sternglanz, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pharmacol, Piscataway, NJ 08854 USA
[2]
A cooperative interaction between U2AF65 and mBBP/SF1 facilitates branchpoint region recognition
[J].
Berglund, JA
;
Abovich, N
;
Rosbash, M
.
GENES & DEVELOPMENT,
1998, 12 (06)
:858-867

Berglund, JA
论文数: 0 引用数: 0
h-index: 0
机构: Brandeis Univ, Howard Hughes Med Inst, Waltham, MA 02254 USA

Abovich, N
论文数: 0 引用数: 0
h-index: 0
机构: Brandeis Univ, Howard Hughes Med Inst, Waltham, MA 02254 USA

Rosbash, M
论文数: 0 引用数: 0
h-index: 0
机构:
Brandeis Univ, Howard Hughes Med Inst, Waltham, MA 02254 USA Brandeis Univ, Howard Hughes Med Inst, Waltham, MA 02254 USA
[3]
Identification of a regulated pathway for nuclear pre-mRNA turnover
[J].
Bousquet-Antonelli, C
;
Presutti, C
;
Tollervey, D
.
CELL,
2000, 102 (06)
:765-775

Bousquet-Antonelli, C
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland

论文数: 引用数:
h-index:
机构:

Tollervey, D
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland Univ Edinburgh, Inst Cell & Mol Biol, Wellcome Trust Ctr Cell Biol, Edinburgh EH9 3JR, Midlothian, Scotland
[4]
Messenger-RNA-binding proteins and the messages they carry
[J].
Dreyfuss, G
;
Kim, VN
;
Kataoka, N
.
NATURE REVIEWS MOLECULAR CELL BIOLOGY,
2002, 3 (03)
:195-205

Dreyfuss, G
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Penn, Sch Med, Howard Hughes Med Inst, Philadelphia, PA 19104 USA Univ Penn, Sch Med, Howard Hughes Med Inst, Philadelphia, PA 19104 USA

Kim, VN
论文数: 0 引用数: 0
h-index: 0
机构: Univ Penn, Sch Med, Howard Hughes Med Inst, Philadelphia, PA 19104 USA

Kataoka, N
论文数: 0 引用数: 0
h-index: 0
机构: Univ Penn, Sch Med, Howard Hughes Med Inst, Philadelphia, PA 19104 USA
[5]
Nuclear architecture and spatial positioning help establish transcriptional states of telomeres in yeast
[J].
Feuerbach, F
;
Galy, V
;
Trelles-Sticken, E
;
Fromont-Racine, M
;
Jacquier, A
;
Gilson, E
;
Olivo-Marin, JC
;
Scherthan, H
;
Nehrbass, U
.
NATURE CELL BIOLOGY,
2002, 4 (03)
:214-221

论文数: 引用数:
h-index:
机构:

Galy, V
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA1773, F-75724 Paris 15, France

Trelles-Sticken, E
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA1773, F-75724 Paris 15, France

论文数: 引用数:
h-index:
机构:

论文数: 引用数:
h-index:
机构:

Gilson, E
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA1773, F-75724 Paris 15, France

Olivo-Marin, JC
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA1773, F-75724 Paris 15, France

Scherthan, H
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA1773, F-75724 Paris 15, France

Nehrbass, U
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA1773, F-75724 Paris 15, France
[6]
Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing
[J].
Gadal, O
;
Strauss, D
;
Petfalski, E
;
Gleizes, PE
;
Gas, N
;
Tollervey, D
;
Hurt, E
.
JOURNAL OF CELL BIOLOGY,
2002, 157 (06)
:941-951

Gadal, O
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany

Strauss, D
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany

Petfalski, E
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany

Gleizes, PE
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany

Gas, N
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany

Tollervey, D
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany

Hurt, E
论文数: 0 引用数: 0
h-index: 0
机构: Biochem Zentrum Heidelberg BZH, D-69120 Heidelberg, Germany
[7]
Nuclear pore complexes in the organization of silent telomeric chromatin
[J].
Galy, V
;
Olivo-Marin, JC
;
Scherthan, H
;
Doye, V
;
Rascalou, N
;
Nehrbass, U
.
NATURE,
2000, 403 (6765)
:108-112

Galy, V
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA 1773, F-75724 Paris 15, France

Olivo-Marin, JC
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA 1773, F-75724 Paris 15, France

Scherthan, H
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA 1773, F-75724 Paris 15, France

Doye, V
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA 1773, F-75724 Paris 15, France

Rascalou, N
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA 1773, F-75724 Paris 15, France

Nehrbass, U
论文数: 0 引用数: 0
h-index: 0
机构: Inst Pasteur, Lab Biol Cellulaire Noyau, CNRS, URA 1773, F-75724 Paris 15, France
[8]
The C-terminal domain of myosin-like protein 1 (Mlp1p) is a docking site for heterogeneous nuclear ribonucleoproteins that are required for mRNA export
[J].
Green, DM
;
Johnson, CP
;
Hagan, H
;
Corbett, AH
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
2003, 100 (03)
:1010-1015

Green, DM
论文数: 0 引用数: 0
h-index: 0
机构:
Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA

Johnson, CP
论文数: 0 引用数: 0
h-index: 0
机构:
Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA

Hagan, H
论文数: 0 引用数: 0
h-index: 0
机构:
Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA

Corbett, AH
论文数: 0 引用数: 0
h-index: 0
机构:
Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA Emory Univ, Sch Med, Dept Biochem, Atlanta, GA 30322 USA
[9]
Quality control of mRNA 3′-end processing is linked to the nuclear exosome
[J].
Hilleren, P
;
McCarthy, T
;
Rosbash, M
;
Parker, R
;
Jensen, TH
.
NATURE,
2001, 413 (6855)
:538-542

Hilleren, P
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Arizona, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA Univ Arizona, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA

McCarthy, T
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA

Rosbash, M
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA

Parker, R
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA

Jensen, TH
论文数: 0 引用数: 0
h-index: 0
机构: Univ Arizona, Howard Hughes Med Inst, Dept Mol & Cellular Biol, Tucson, AZ 85721 USA
[10]
A U5 SMALL NUCLEAR RIBONUCLEOPROTEIN PARTICLE PROTEIN INVOLVED ONLY IN THE 2ND STEP OF PREMESSENGER RNA SPLICING IN SACCHAROMYCES-CEREVISIAE
[J].
HOROWITZ, DS
;
ABELSON, J
.
MOLECULAR AND CELLULAR BIOLOGY,
1993, 13 (05)
:2959-2970

HOROWITZ, DS
论文数: 0 引用数: 0
h-index: 0
机构:
CALTECH,DIV BIOL 14775,PASADENA,CA 91125 CALTECH,DIV BIOL 14775,PASADENA,CA 91125

ABELSON, J
论文数: 0 引用数: 0
h-index: 0
机构:
CALTECH,DIV BIOL 14775,PASADENA,CA 91125 CALTECH,DIV BIOL 14775,PASADENA,CA 91125