Making mitochondrial mutants

被引：16

作者：

Jacobs, HT ^{[1
]}

机构：

[1] Tampere Univ, Inst Med Technol, FIN-33014 Tampere, Finland

[2] Tampere Univ, Tampere Univ Hosp, FIN-33014 Tampere, Finland

来源：

TRENDS IN GENETICS | 2001年 / 17卷 / 11期

基金：

芬兰科学院;

关键词：

D O I：

10.1016/S0168-9525(01)02480-5

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

Mitochondrial DNA (mtDNA) encodes a mere 13 polypeptides, all with well-defined cellular functions in mitochondrial energy metabolism. It was first sequenced over two decades ago, yet our understanding of the wider physiological role of mtDNA is surprisingly sketchy. Partly, this reflects the fact that the mitochondrial gene products are essential for life; that is, most mtDNA mutations are expected to be lethal. The technical difficulty of engineering mtDNA mutations has been a major handicap in furthering our understanding of the mitochondrial genetic system. Recent developments now offer some possibilities for the genetic manipulation of mtDNA and for elucidating its contribution to human development, physiology and disease.

引用

页码：653 / 660

页数：8

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