A2rel:: a constitutively expressed Leishmania gene linked to an amastigote-stage-specific gene

被引：14

作者：

Ghedin, E

Charest, H

Matlashewski, G

机构：

[1] McGill Univ, Inst Parasitol, St Anne De Bellevue, PQ H9X 3V9, Canada

[2] NIAID, Parasit Dis Lab, Immunobiol Sect, NIH, Bethesda, MD 20892 USA

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1998年 / 93卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1016/S0166-6851(98)00027-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The A2-A2rel gene copies are arranged in tandem arrays on a 850 kb chromosome in Leishmania donovani. Contrary to A2 mRNA which displays amastigote-stage-specific expression, A2rel gene expression is constitutive throughout the L. donovani life cycle. The A2rel sequence was found to be conserved in all Leishmania species tested, while the A2 sequence is specific to L. donovani and L. mexicana. The A2rel full length cDNA is of 2.3 kb and it contains one open reading frame coding for a putative protein of 436 amino acids. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

收藏

页码：23 / 29

页数：7

相关论文

共 13 条

[1] MULTIPLICATION OF A HUMAN PARASITE (LEISHMANIA-DONOVANI) IN PHAGOLYSOSOMES OF HAMSTER MACROPHAGES INVITRO [J].

CHANG, KP ;

DWYER, DM .

SCIENCE, 1976, 193 (4254) :678-680

[2] The developmental expression of Leishmania donovani A2 amastigote-specific genes is post-transcriptionally mediated and involves elements located in the 3'-untranslated region [J].

Charest, H ;

Zhang, WW ;

Matlashewski, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (29) :17081-17090

[3] DEVELOPMENTAL GENE-EXPRESSION IN LEISHMANIA-DONOVANI - DIFFERENTIAL CLONING AND ANALYSIS OF AN AMASTIGOTE-STAGE-SPECIFIC GENE [J].

CHAREST, H ;

MATLASHEWSKI, G .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (05) :2975-2984

[4] Antibody response against a Leishmania donovani amastigote-stage-specific protein in patients with visceral leishmaniasis [J].

Ghedin, E ;

Zhang, WW ;

Charest, H ;

Sundar, S ;

Kenney, RT ;

Matlashewski, G .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1997, 4 (05) :530-535

[5] CLONING AND CHARACTERIZATION OF DIFFERENTIALLY EXPRESSED GENES FROM INVITRO-GROWN AMASTIGOTES OF LEISHMANIA-DONOVANI [J].

JOSHI, M ;

DWYER, DM ;

NAKHASI, HL .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1993, 58 (02) :345-354

[6] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[7] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[8] PREDICTION OF TRANSMEMBRANE SEGMENTS IN PROTEINS UTILIZING MULTIPLE SEQUENCE ALIGNMENTS [J].

PERSSON, B ;

ARGOS, P .

JOURNAL OF MOLECULAR BIOLOGY, 1994, 237 (02) :182-192

[9] IDENTIFICATION OF DIFFERENTIALLY EXPRESSED LEISHMANIA-DONOVANI GENES USING ARBITRARILY PRIMED POLYMERASE CHAIN-REACTIONS [J].

POGUE, GP ;

LEE, NS ;

KOUL, S ;

DWYER, DM ;

NAKHASI, HL .

GENE, 1995, 165 (01) :31-38

[10]

VONHEIJNE G, 1983, EUR J BIOCHEM, V133, P17