RNAi screens in Caenorhabditis elegans in a 96-well liquid format and their application to the systematic identification of genetic interactions

被引：95

作者：

Lehner, Ben

Tischler, Julia

Fraser, Andrew G. ^{[1
]}

机构：

[1] Wellcome Trust Sanger Inst, Cambridge CB10 1HH, England

[2] CRG EMBL Syst Biol Program, Ctr Genom Regulat, Barcelona 08003, Spain

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/nprot.2006.245

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a protocol for performing RNA interference (RNAi) screens in Caenorhabditis elegans in liquid culture in 96-well plates. The procedure allows a single researcher to set-up and score RNAi experiments at similar to 2,000 genes per day. By comparing RNAi phenotypes between wild-type worms and worms carrying a defined genetic mutation, we have used this protocol to identify synthetic lethal interactions between genes systematically. We also describe how the protocol can be adapted to target two genes simultaneously by combinatorial RNAi.

引用

收藏

页码：1617 / 1620

页数：4

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