Developmental changes of nitric oxide-sensitive guanylyl cyclase expression in pulmonary arteries

被引:32
作者
Behrends, S
Kempfert, J
Mietens, A
Koglin, M
Scholz, H
Middendorff, R
机构
[1] Univ Hamburg, Clin Eppendorf, Inst Pharmakol, D-20246 Hamburg, Germany
[2] Univ Hamburg, Inst Anat, D-20246 Hamburg, Germany
关键词
nitric oxide; soluble guanylyl cyclase; endothelium; smooth muscle cell; pulmonary vasculature; development;
D O I
10.1006/bbrc.2001.4869
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inhaled nitric oxide (NO) is known to influence the contractile state of pulmonary arteries most likely by activation of soluble guanylyl cyclase (sGC) in smooth muscle cells. However, the cellular distribution of sGC has not been determined empirically, due to a lack of specific antibodies. Here, we describe a novel antibody directed against the beta (1) subunit of sGC to study the cellular distribution of sGC in lung during development. Using the novel antibody, the enzyme was demonstrated in fetal, neonatal, and adult lungs by Western blot, showing maximum expression in neonatal lung. These data were confirmed by measurements of sGC activity. In pulmonary arteries of fetal lung sGC-beta (1) immunoreactivity was present in smooth muscle cells and absent in endothelial cells. With postnatal development an increase in immunoreactivity in endothelial cells and a reciprocal decrease in smooth muscle cells was apparent. The reported changes in sGC expression likely contribute to the known age-dependent differences in response to inhaled NO. (C) 2001 Academic Press.
引用
收藏
页码:883 / 887
页数:5
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