Applications of Loop-Mediated Isothermal DNA Amplification

被引：137

作者：

Fu, Shijun ^{[1
,2
]}

Qu, Guanggang ^{[2
,3
]}

Guo, Shijin ^{[1
,2
]}

Ma, Lin ^{[1
]}

Zhang, Na ^{[3
]}

Zhang, Songlin ^{[2
]}

Gao, Sanyang ^{[2
,3
]}

Shen, Zhiqiang ^{[1
,2
,3
]}

机构：

[1] Shandong Lvdu Ante Vet Drug Ind Co Ltd, Binzhou 256600, Peoples R China

[2] Binzhou Anim Husb & Vet Res Acad, Key Lab Prevent Vet Med & Anim Biotechnol, Binzhou 256600, Peoples R China

[3] Shandong Lvdu Biol Technol Co Ltd, Binzhou 256600, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2011年 / 163卷 / 07期

关键词：

Loop-mediated isothermal amplification; Pathogenic microorganisms; Detection; Application; RAPID DETECTION; PARACOCCIDIOIDES-BRASILIENSIS; CAMPYLOBACTER-JEJUNI; SENSITIVE DETECTION; DIAGNOSIS; VIRUS; LAMP; ASSAY; GENE; FISH;

D O I：

10.1007/s12010-010-9088-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.

引用

页码：845 / 850

页数：6

共 41 条

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