Role for piRNAs and Noncoding RNA in de Novo DNA Methylation of the Imprinted Mouse Rasgrf1 Locus

被引：293

作者：

Watanabe, Toshiaki ^{[1
,2
,3
]}

Tomizawa, Shin-ichi ^{[1
,2
,4
]}

Mitsuya, Kohzoh ^{[5
]}

Totoki, Yasushi ^{[6
]}

Yamamoto, Yasuhiro ^{[1
,2
,7
]}

Kuramochi-Miyagawa, Satomi ^{[8
]}

Iida, Naoko ^{[1
,2
,9
]}

Hoki, Yuko ^{[1
,2
,7
]}

Murphy, Patrick J. ^{[10
]}

Toyoda, Atsushi ^{[11
]}

Gotoh, Kengo ^{[8
]}

Hiura, Hitoshi ^{[12
]}

Arima, Takahiro ^{[12
]}

Fujiyama, Asao ^{[11
,13
]}

Sado, Takashi ^{[1
,2
,7
]}

Shibata, Tatsuhiro ^{[6
]}

Nakano, Toru ^{[8
]}

Lin, Haifan ^{[3
]}

Ichiyanagi, Kenji ^{[1
,2
,7
]}

Soloway, Paul D. ^{[10
]}

Sasaki, Hiroyuki ^{[1
,2
,7
]}

机构：

[1] Res Org Informat & Syst, Natl Inst Genet, Div Human Genet, Mishima, Shizuoka 4118540, Japan

[2] Res Org Informat & Syst, Natl Inst Genet, Dept Integrated Genet, Mishima, Shizuoka 4118540, Japan

[3] Yale Univ, Sch Med, New Haven, CT 06520 USA

[4] Babraham Inst, Lab Dev Genet & Imprinting, Cambridge CB22 3AT, England

[5] Univ Texas Hlth Sci Ctr San Antonio, Dept Obstet & Gynecol, Ctr Pregnancy & Newborn Res, San Antonio, TX 78229 USA

[6] Natl Canc Ctr, Res Inst, Div Canc Genom, Chuo Ku, Tokyo 1040045, Japan

[7] Kyushu Univ, Med Inst Bioregulat, Div Epigenom, Higashi Ku, Fukuoka 8128582, Japan

[8] Osaka Univ, Grad Sch Frontier Biosci, Suita, Osaka 5650871, Japan

[9] Res Org Informat & Syst, Natl Inst Genet, Dept Mol Genet, Div Mutagenesis, Mishima, Shizuoka 4118540, Japan

[10] Cornell Univ, Coll Agr & Life Sci, Div Nutr Sci, Ithaca, NY 14853 USA

[11] Res Org Informat & Syst, Natl Inst Genet, Ctr Genet Resource Informat, Comparat Genom Lab, Mishima, Shizuoka 4118540, Japan

[12] Tohoku Univ, Grad Sch Med, Environm & Genome Res Ctr, Dept Informat Genet,Aoba Ku, Sendai, Miyagi 9808575, Japan

[13] Natl Inst Informat, Principle Informat Res Div, Chiyoda Ku, Tokyo 1018430, Japan

来源：

SCIENCE | 2011年 / 332卷 / 6031期

关键词：

GERM-CELL DEVELOPMENT; MAMMALIAN DEVELOPMENT; DROSOPHILA; TRANSCRIPTION; FAMILY; GENES; PIWI; MILI;

D O I：

10.1126/science.1203919

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Genomic imprinting causes parental origin-specific monoallelic gene expression through differential DNA methylation established in the parental germ line. However, the mechanisms underlying how specific sequences are selectively methylated are not fully understood. We have found that the components of the PIWI-interacting RNA (piRNA) pathway are required for de novo methylation of the differentially methylated region (DMR) of the imprinted mouse Rasgrf1 locus, but not other paternally imprinted loci. A retrotransposon sequence within a noncoding RNA spanning the DMR was targeted by piRNAs generated from a different locus. A direct repeat in the DMR, which is required for the methylation and imprinting of Rasgrf1, served as a promoter for this RNA. We propose a model in which piRNAs and a target RNA direct the sequence-specific methylation of Rasgrf1.

引用

页码：848 / 852

页数：5

共 29 条

[1] A piRNA pathway primed by individual transposons is linked to de novo DNA methylation in mice [J].