PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.-specific kinetoplastid primers

被引：124

作者：

Cortes, S ^{[1
]}

Rolao, N ^{[1
]}

Ramada, J ^{[1
]}

Campino, L ^{[1
]}

机构：

[1] Univ Nova Lisboa, IHMT, Ctr Malaria Outras Doencas Tropicais, Unidade Leishmanioses, P-1349008 Lisbon, Portugal

来源：

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE | 2004年 / 98卷 / 01期

关键词：

leishmaniasis; Leishmania donovoni s.l; polymerase chain reaction; kinetoplastid primers;

D O I：

10.1016/S0035-9203(03)00002-6

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

This study was performed in order to test the efficacy of a new polymerase chain reaction (PCR) assay for the diagnosis of both human and canine leishmaniasis caused by Leishmania infanturn. The new primers were developed on the basis of a complete DNA sequence of the L. infantum kinetoptast minicircle. Specificity and sensitivity were evaluated by testing bone marrow spots on fitter paper and skin biopsy samples, and the PCR results were compared to data from in vitro cultures. Leishmania strains from different foci, as well as other trypanosomatids and opportunistic pathogenic micro-organisms, were also included in this study. The results show that the primers are highly specific, detecting only L. donovani s.l. DNA, and sensitive for the detection of parasite DNA in biological samples from three different geographical regions of Portugal (north, centre and south) and from Brazil. (C) 2003 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.

引用

页码：12 / 17

页数：6

共 23 条

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