Manipulating crystals with light

被引:9
作者
Bancel, PA
Cajipe, VB
Rodier, F
机构
[1] CNRS UMR C6502, Inst Mat Nantes, Chim Solide Lab, F-44322 Nantes 3, France
[2] CNRS UMR C6502, Inst Mat Nantes, Lab Phys Cristalline, F-44322 Nantes, France
[3] CNRS, Lab Enzymol & Biochim Struct, F-91198 Gif Sur Yvette, France
关键词
optical trapping; protein crystallization; microcrystals; seeding;
D O I
10.1016/S0022-0248(98)00877-X
中图分类号
O7 [晶体学];
学科分类号
0702 ; 070205 ; 0703 ; 080501 ;
摘要
When light is scattered by an object there is an exchange of momentum which results in a net force being applied to the scatterer. Although relatively weak, optical forces can be significant for micro-objects in intense light fields. For example, milliwatts of laser power generate optical forces sufficient to levitate and transport 10 mu m particles. Applied to biological matter, optical force techniques offer the important advantage of permitting contactless, nonmechanical, in situ manipulation. By appropriate selection of the laser wavelength, radiation and thermal damage can be avoided. We describe a compact laser seeding apparatus by which individual crystal micro-seeds can be selected and transferred into a growth solution. The device allows for the rapid, reproducible transfer of single seeds. Other plausible uses of the seeding apparatus are seeding directly into capillary tubes and transfer of microcrystals into cryo-loops. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:685 / 690
页数:6
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