Quantitation and purification of polymerase chain reaction products by high-performance liquid chromatography

被引：5

作者：

Katz, ED ^{[1
]}

机构：

[1] PERKIN ELMER CORP,NORWALK,CT 06859

来源：

MOLECULAR BIOTECHNOLOGY | 1996年 / 6卷 / 01期

关键词：

polymerase chain reaction; HPLC; anion-exchange chromatography; PCR product quantitation;

D O I：

10.1007/BF02762327

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work describes the application of the fully:automated high-performance liquid chromatographic system to the analysis of PCR-amplified products. Efficient separations of both DNA restriction fragments and PCR products were performed using an anion-exchange DEAE-NPR column, packed with 2.5-mu m nonporous particles. The automated HPLC method was employed for the separation, quantitation, and purification of PCR products in less than 10 min in a single step.

引用

页码：79 / 86

页数：8

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