HTP-1 coordinates synaptonemal complex assembly with homolog alignment during meiosis in C-elegans

被引:110
作者
Couteau, F [1 ]
Zetka, M [1 ]
机构
[1] McGill Univ, Dept Biol, Montreal, PQ H3A 1B1, Canada
关键词
meiosis; synapsis; homolog alignment; recombination; C; elegans;
D O I
10.1101/gad.1348205
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
During meiosis, the mechanisms responsible for homolog alignment, synapsis, and recombination are precisely coordinated to culminate in the formation of crossovers capable of directing accurate chromosome segregation. An outstanding question is how the cell ensures that the structural hallmark of meiosis, the synaptonemal complex (SC), forms only between aligned pairs of homologous chromosomes. In the present study, we find that two closely related members of the him-3 gene family in Caenorhabditis elegans function as regulators of synapsis. HTP-1 functionally couples homolog alignment to its stabilization by synapsis by preventing the association of SC components with unaligned and immature chromosome axes; in the absence of the protein, nonhomologous contacts between chromosomes are inappropriately stabilized, resulting in extensive nonhomologous synapsis and a drastic decline in chiasma formation. In the absence of both HTP-1 and HTP-2, synapsis is abrogated per se and the early association of SC components with chromosomes observed in htp-1 mutants does not occur, suggesting a function for the proteins in licensing SC assembly. Furthermore, our results suggest that early steps of recombination occur in a narrow window of opportunity in early prophase that ends with SC assembly, resulting in a mechanistic coupling of the two processes to promote crossing over.
引用
收藏
页码:2744 / 2756
页数:13
相关论文
共 42 条
[1]   The HORMA domain: a common structural denominator in mitotic checkpoints, chromosome synapsis and DNA repair [J].
Aravind, L ;
Koonin, EV .
TRENDS IN BIOCHEMICAL SCIENCES, 1998, 23 (08) :284-286
[2]  
Bhuiyan H, 2003, GENETICS, V163, P539
[3]  
BRENNER S, 1974, GENETICS, V77, P71
[4]   Synaptonemal complex assembly in C-elegans is dispensable for loading strand-exchange proteins but critical for proper completion of recombination [J].
Colaiácovo, MP ;
MacQueen, AJ ;
Martinez-Perez, E ;
McDonald, K ;
Adamo, A ;
La Volpe, A ;
Villeneuve, AM .
DEVELOPMENTAL CELL, 2003, 5 (03) :463-474
[5]   A component of C-elegans meiotic chromosome axes at the interface of homolog alignment, synapsis, nuclear reorganization, and recombination [J].
Couteau, F ;
Nabeshima, K ;
Villeneuve, A ;
Zetka, M .
CURRENT BIOLOGY, 2004, 14 (07) :585-592
[6]   CONFIDENCE INTERVALS FOR THE EXPECTATION OF A POISSON VARIABLE [J].
CROW, EL ;
GARDNER, RS .
BIOMETRIKA, 1959, 46 (3-4) :441-453
[7]   Meiotic recombination in C-elegans initiates by a conserved mechanism and is dispensable for homologous chromosome synapsis [J].
Dernburg, AF ;
McDonald, K ;
Moulder, G ;
Barstead, R ;
Dresser, M ;
Villeneuve, AM .
CELL, 1998, 94 (03) :387-398
[8]   Organization of the yeast Zip1 protein within the central region of the synaptonemal complex [J].
Dong, HJ ;
Roeder, GS .
JOURNAL OF CELL BIOLOGY, 2000, 148 (03) :417-426
[9]   Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].
Fire, A ;
Xu, SQ ;
Montgomery, MK ;
Kostas, SA ;
Driver, SE ;
Mello, CC .
NATURE, 1998, 391 (6669) :806-811
[10]  
FRANCIS R, 1995, GENETICS, V139, P579