Role of modulator recognition factor 2 in adipogenesis and leptin expression in 3T3-L1 cells

被引：8

作者：

Dong, Jie ^{[2
]}

Ishimori, Naoki ^{[2
]}

Paigen, Beverly ^{[3
]}

Tsutsui, Hiroyuki ^{[2
]}

Fujii, Satoshi ^{[1
,2
]}

机构：

[1] Nagoya City Univ, Grad Sch Pharmaceut Sci, Dept Mol & Cellular Pathobiol & Therapeut, Mizuho Ku, Nagoya, Aichi 4678603, Japan

[2] Hokkaido Univ, Grad Sch Med, Dept Cardiovasc Med, Sapporo, Hokkaido, Japan

[3] Jackson Lab, Bar Harbor, ME 04609 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 366卷 / 02期

关键词：

adipocyte; adipokine; differentiation; insulin; leptin; modulator recognition factor;

D O I：

10.1016/j.bbrc.2007.12.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The complex network of adipogenic transcription factors regulates adipocyte differentiation, obesity, and insulin resistance. Modulator recognition factor (Mrf) 2 knockout mice exhibit defects in fat accumulation and are protected from diet-induced obesity, suggesting that Mrf2 deficiency affects adipogenesis. Here, we report that the gene expressions of the 2 isoforms of the transcription factors Mrf2, Mrf2 alpha, and Mrf2 beta, were induced upon adipogenesis in 3T3-L1 cells. Mrf2 mRNA expression was sensitive to stimulation by insulin, dexamethasone, and TNF-alpha in 3T3-L1 preadipocytes and differentiated adipocytes. Down-regulation of Mrf2 alpha and Mrf2 beta gene expressions induced by small interfering RNAs increased the mRNA expression of leptin. These results indicate that Mrf2 can be a potential regulator of adipocyte differentiation and a potential repressor of leptin. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：551 / 555

页数：5

共 23 条

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