Proteinase inhibitor accumulation in aphid-infested barley leaves

被引:34
作者
Casaretto, JA
Corcuera, LJ [1 ]
机构
[1] Univ Chile, Fac Ciencias, Dept Biol, Santiago, Chile
[2] Univ Concepcion, Dept Bot, Concepcion, Chile
关键词
Hordeum vulgare; Gramineae; Schizaphis graminum; Rhopalosiphum padi; aphids; defense proteins;
D O I
10.1016/S0031-9422(98)00332-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Proteinase inhibitors (chymotrypsin and trypsin inhibitors) accumulation in barley leaves was studied when the plants were infested with the aphids Schizaphis graminum and Rhopalosiphum padi. Aphid infestation resulted mainly in the accumulation of chymotrypsin inhibitors (about two fold increase) and this response was higher with S. graminum. Frontera, a cultivar which was more resistant to aphids, accumulated more inhibitors than other cultivars. Compounds responsible for the inhibitory activities were separated by gel filtration chromatography. The separation revealed two peaks of chymotrypsin inhibitors and one peak of trypsin inhibitor. M-r estimations for these proteins indicated sizes Similar to those inhibitors found in barley seeds. These inhibitors had no effect on survival of S. graminum reared on artificial diets, but decreased survival of R. padi. (C) 1998 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2279 / 2286
页数:8
相关论文
共 30 条
[1]   APHID FEEDING AND NUTRITION [J].
AUCLAIR, JL .
ANNUAL REVIEW OF ENTOMOLOGY, 1963, 8 :439-+
[2]   THIONINS [J].
BOHLMANN, H ;
APEL, K .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1991, 42 :227-240
[3]   INHIBITORS OF CHYMOTRYPSIN AND MICROBIAL SERINE PROTEASES IN BARLEY GRAINS - ISOLATION, PARTIAL CHARACTERIZATION AND IMMUNOCHEMICAL RELATIONSHIPS OF MULTIPLE MOLECULAR-FORMS [J].
BOISEN, S ;
ANDERSEN, CY ;
HEJGAARD, J .
PHYSIOLOGIA PLANTARUM, 1981, 52 (02) :167-176
[4]   PROTEASE INHIBITOR FROM BARLEY EMBRYO INHIBITING TRYPSIN AND TRYPSIN-LIKE MICROBIAL PROTEASES - PURIFICATION AND CHARACTERIZATION OF 2 ISOFORMS [J].
BOISEN, S ;
DJURTOFT, R .
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1982, 33 (05) :431-440
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   CHANGES IN FERULIC ACID AND LIPID-CONTENT IN APHID-INFESTED BARLEY [J].
CABRERA, HM ;
MUNOZ, O ;
ZUNIGA, GE ;
CORCUERA, LJ ;
ARGANDONA, VH .
PHYTOCHEMISTRY, 1995, 39 (05) :1023-1026
[7]  
Casaretto Jose A., 1995, Biological Research, V28, P239
[8]   BIOCHEMICAL BASIS FOR THE RESISTANCE OF BARLEY TO APHIDS [J].
CORCUERA, LJ .
PHYTOCHEMISTRY, 1993, 33 (04) :741-747
[9]   EXPRESSION OF A MAIZE PROTEINASE-INHIBITOR GENE IS INDUCED IN RESPONSE TO WOUNDING AND FUNGAL INFECTION - SYSTEMIC WOUND-RESPONSE OF A MONOCOT GENE [J].
CORDERO, MJ ;
RAVENTOS, D ;
SANSEGUNDO, B .
PLANT JOURNAL, 1994, 6 (02) :141-150
[10]   PERMANENT CULTURE OF AN APHID ON A TOTALLY SYNTHETIC DIET [J].
DADD, RH ;
MITTLER, TE .
EXPERIENTIA, 1966, 22 (12) :832-&