Heat acclimation and cross-tolerance against anoxia in Arabidopsis

被引:75
作者
Banti, Valeria [2 ]
Loreti, Elena [3 ]
Novi, Giacomo [1 ]
Santaniello, Antonietta [1 ]
Alpi, Amedeo [2 ]
Perata, Pierdomenico [1 ]
机构
[1] Scuola Super Sant Anna, Plant & Crop Physiol Lab, I-56124 Pisa, Italy
[2] Univ Pisa, Dept Crop Plant Biol, I-56124 Pisa, Italy
[3] CNR, Ist Biol & Biotecnol Agr, I-56100 Pisa, Italy
关键词
heat shock; hypoxia; sucrose;
D O I
10.1111/j.1365-3040.2008.01816.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Arabidopsis seedlings are highly sensitive to low oxygen and they die rapidly when exposed to anoxia. Tolerance to anoxia depends on the ability to efficiently use carbohydrates through the fermentative pathway, as highlighted by the lower tolerance displayed by a mutant devoid of alcohol dehydrogenase. Other mechanisms of tolerance are also possible and may include a role for heat-induced genes. In fact, heat shock proteins (HSPs) are induced by anoxia. This suggests that there may be a cross-adaptation mechanism between heat and anoxic stress, and in this work, we studied the acclimation of Arabidopsis seedlings both to low oxygen and heat. The results show that seedlings subjected to hypoxia or heat pretreatment survive anoxia much better. Interestingly, we also observed an increased anoxia tolerance in heat-treated alcohol dehydrogenase (adh) mutant plants. On the other hand, anoxic pretreatment does not confer tolerance to heat stress. The success of the induction of HSPs by anoxia is in direct relation to the amount of sucrose available, and this in turn relates to how well seedlings will survive under anoxia. HSP transcripts were also detected during seed development and germination, two hypoxia-prone processes, suggesting that hypoxia-induced HSP expression is physiologically relevant.
引用
收藏
页码:1029 / 1037
页数:9
相关论文
共 38 条
[1]   EFFECTS OF O-2 CONCENTRATION ON RICE SEEDLINGS [J].
ALPI, A ;
BEEVERS, H .
PLANT PHYSIOLOGY, 1983, 71 (01) :30-34
[2]  
Bernst E, 1974, METHOD ENZYMAT AN, P1499
[3]   Anoxic stress leads to hydrogen peroxide formation in plant cells [J].
Blokhina, OB ;
Chirkova, TV ;
Fagerstedt, KV .
JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 (359) :1179-1190
[4]   Isolation of arabidopsis mutants lacking components of acquired thermotolerance [J].
Burke, JJ ;
O'Mahony, PJ ;
Oliver, MJ .
PLANT PHYSIOLOGY, 2000, 123 (02) :575-587
[5]   Expression of heat shock proteins in turtle and mammal hearts: relationship to anoxia tolerance [J].
Chang, J ;
Knowlton, AA ;
Wasser, JS .
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 2000, 278 (01) :R209-R214
[6]   Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants [J].
Couée, I ;
Sulmon, C ;
Gouesbet, G ;
El Amrani, A .
JOURNAL OF EXPERIMENTAL BOTANY, 2006, 57 (03) :449-459
[7]   POLYMORPHISM OF ALCOHOL-DEHYDROGENASE IN ARABIDOPSIS-THALIANA (L) HEYNH - GENETIC AND BIOCHEMICAL-CHARACTERIZATION [J].
DOLFERUS, R ;
JACOBS, M .
BIOCHEMICAL GENETICS, 1984, 22 (9-10) :817-838
[8]   Arabidopsis roots and shoots have different mechanisms for hypoxic stress tolerance [J].
Ellis, MH ;
Dennis, ES ;
Peacock, WJ .
PLANT PHYSIOLOGY, 1999, 119 (01) :57-64
[9]   Plant responses to hypoxia - is survival a balancing act? [J].
Fukao, T ;
Bailey-Serres, J .
TRENDS IN PLANT SCIENCE, 2004, 9 (09) :449-456
[10]   Response of plant metabolism to too little oxygen [J].
Geigenberger, P .
CURRENT OPINION IN PLANT BIOLOGY, 2003, 6 (03) :247-256