Novel phosphotransferase-encoding genes revealed by analysis of the Escherichia coli genome: A chimeric gene encoding an Enzyme I homologue that possesses a putative sensory transduction domain

被引：63

作者：

Reizer, J

Reizer, A

Merrick, MJ

Plunkett, G

Rose, DJ

Saier, MH

机构：

[1] JOHN INNES CTR PLANT SCI RES,NITROGEN FIXAT LAB,NORWICH NR4 7UH,NORFOLK,ENGLAND

[2] UNIV WISCONSIN,GENET LAB,MADISON,WI 53706

来源：

GENE | 1996年 / 181卷 / 1-2期

关键词：

E-coli; genomephosphotransferase system; nitrogen assimilation; protein phosphorylation; sugar transport;

D O I：

10.1016/S0378-1119(96)00481-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two genes (ptsI and ptsA) that encode homologues of the energy coupling Enzyme I of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system (PTS) have previously been identified on the Escherichia coli chromosome. We here report the presence of a third E. coli gene, designated ptsP, that encodes an Enzyme I homologue, here designated Enzyme I-Ntr Enzyme I-Ntr possesses an N-terminal domain homologous to the N-terminal domains of NifA proteins [(127 amino acids (aa)] joined via two tandem flexible linkers to the C-terminal Enzyme I-like domain (578 aa). Structural features of the putative ptsP operon, including transcriptional regulatory signals, are characterized. We suggest that Enzyme I-Ntr functions in transcriptional regulation of nitrogen-related operons together with previously described PTS proteins encoded within the rpoN operon. It may thereby provide a link between carbon and nitrogen assimilatory pathways.

引用

页码：103 / 108

页数：6

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