Kup is the major K+ uptake system in Escherichia coli upon hyper-osmotic stress at a low pH

The K+ uptake was observed in washed cells of Escherichia coli, wild-type, upon hyper-osmotic stress at pH 5.5 when glucose was supplemented, This uptake had apparent a K-m of 0.58 mM and V-max of 0.10 mu mol K+/min/mg protein. Such a K+ uptake was investigated using a mutant defective in Kdp and TrkA but with a functional Kup and a mutant defective in Kdp and Kup but having an active TrkA, The K+ uptake to reach the steady state level as well as the initial K+ influx rate in the first mutant were at least 3.5-fold greater than these values with the second mutant and similar to those of the wild-type, Such differences in the K+ uptake activity were correlated with K+ requirements for growth of these mutants, Moreover, the K+ uptake in the wild-type was blocked by a protonophore (carbonyl cyanide m-chlorophenylhydrazone). Valinomycin, arsenate and N,N'-dicyclohexylcarbodiimide were not effective in changing the K+ uptake, It is suggested that Kup is the major K+ uptake system in E, coli upon hyper-osmotic stress at a low pH. (C) 1999 Federation of European Biochemical Societies.