Effect of smokeless tobacco extract on human gingival keratinocyte levels of prostaglandin E(2) and interleukin-1

被引:36
作者
Johnson, GK
Poore, TK
Payne, JB
Organ, CC
机构
[1] UNIV IOWA,COLL DENT,DOWS INST DENT RES,IOWA CITY,IA 52242
[2] UNIV NEBRASKA,MED CTR,COLL DENT,DEPT SURG SPECIALTIES,LINCOLN,NE 68583
关键词
tobacco; smokeless adverse effects; gingival recession etiology; mucosal diseases etiology; keratinocytes metabolism; prostaglandin; interleukin-1;
D O I
10.1902/jop.1996.67.2.116
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
GINGIVAL RECESSION AND WHITE MUCOSAL lesions frequently occur at sites of smokeless tobacco (ST) placement. The etiology of these alterations is presumably related to the irritating effects of tobacco components. The purpose of this study was to examine the effect of an aqueous ST extract (STE) on gingival keratinocyte production of prostaglandin E(2) (PGE(2)) and interleukin-1 (IL-1), mediators involved in periodontal destruction and keratinocyte proliferation. Keratinocyte cultures were established from healthy tissues discarded from 8 subjects undergoing crown lengthening procedures. Cells (passage 2-3) were seeded at 2.5 x 10(4) cells/well into 48 well tissue culture plates and maintained in serum-free media at 37 degrees C. On day 4 or 5, the wells were divided into 4 groups receiving either 10%, 5%, 2.5%, or 0% STE for time periods ranging from 30 to 240 minutes. PGE(2) levels (pg/10(4) cells), as measured by enzyme immunoassay, were significantly (P < 0.05) increased in the 10% (215.66 +/- 34.58) and 5% STE (151.82 +/- 27.97) treated cultures compared to untreated cells (46.16 +/- 9.58). IL-1 alpha and IL-1 beta proteins were elevated (P < 0.05) in cell lysates (299.45 +/- 38.69 and 28.45 +/- 5.18, respectively) from 5% STE exposed cultures compared to control wells. At 10% STE, secreted IL-1 alpha was decreased (P < 0.05) relative to 2.5% STE. This may reflect a toxic effect, as 10% STE significantly (P < 0.05) depressed cell numbers and viability. Lower tobacco concentrations did not affect cell numbers or viability, but significantly (P < 0.05) increased PGE(2) and IL-1 levels. Tobacco-induced synthesis of these mediators may play a role in the development of tobacco-related oral disease.
引用
收藏
页码:116 / 124
页数:9
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