7-Methylguanosine at the anticodon wobble position of squid mitochondrial tRNASerGCU:: molecular basis for assignment of AGA/AGG codons as serine in invertebrate mitochondria

被引：33

作者：

Tomita, K ^{[1
]}

Ueda, T ^{[1
]}

Watanabe, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, Tokyo 1138656, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1399卷 / 01期

关键词：

tRNA(Ser)GCU; mitochondrion; decoding of AGA and AGG codons; 7-methylguanosine; (squid; Loligo bleekeri);

D O I：

10.1016/S0167-4781(98)00099-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In mitochondria of the squid, Loligo bleekeri, both the AGA and AGG codons are considered to correspond to serine instead of arginine as in the universal genetic code, and its genome encodes a single tRNA(Ser) gene with the anticodon GCT. Therefore, this gene product, tRNA(Ser)GCU, should be able to translate all four AGN (N; U, C, A, and G) codons as serine. To elucidate this recognition mechanism, the tRNA(Ser)GCU was isolated from squid liver and its complete nucleotide sequence determined. The tRNA(Ser)GCU was found to possess 7-methylguanosine (m(7)G) at the wobble position of the anticodon. This suggests that in the squid mitochondrial system, tRNA(Ser)GCU with the anticodon m(7)GCU can recognize not only the usual serine codons AGU and AGC, but also the unusual serine codons AGA and AGG, as in the case of starfish mitochondria (Matsuyama et al., J. Biol. Chem. 273 (1985) 3363-3368). (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：78 / 82

页数：5

共 21 条

[1]

Beard C.B., 1993, Insect Molecular Biology, V2, P103, DOI 10.1111/j.1365-2583.1993.tb00131.x

[2] THE MITOCHONDRIAL-DNA MOLECULE OF DROSOPHILA-YAKUBA - NUCLEOTIDE-SEQUENCE, GENE ORGANIZATION, AND GENETIC-CODE [J].

CLARY, DO ;

WOLSTENHOLME, DR .

JOURNAL OF MOLECULAR EVOLUTION, 1985, 22 (03) :252-271

[3]

CROZIER RH, 1993, GENETICS, V133, P97

[4] PHY M - AN RNASE ACTIVITY SPECIFIC FOR U-RESIDUES AND A-RESIDUES USEFUL IN RNA SEQUENCE-ANALYSIS [J].

DONISKELLER, H .

NUCLEIC ACIDS RESEARCH, 1980, 8 (14) :3133-+

[5] SEQUENCE AND STRUCTURE OF A SERINE TRANSFER-RNA WITH GCU ANTICODON FROM MOSQUITO MITOCHONDRIA [J].

DUBIN, DT ;

HSUCHEN, CC ;

CLEAVES, GR ;

TIMKO, KD .

JOURNAL OF MOLECULAR BIOLOGY, 1984, 176 (02) :251-260

[6] ROLE OF TRANSFER-RNA MODIFICATION IN TRANSLATIONAL FIDELITY [J].

HAGERVALL, TG ;

ERICSON, JU ;

ESBERG, KB ;

LI, JN ;

BJORK, GR .

BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1050 (1-3) :263-266

[7] TRYPTOPHAN TRANSFER RNA AS UGA SUPPRESSOR [J].

HIRSH .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 58 (02) :439-&

[8]

KUCHINO Y, 1987, METHOD ENZYMOL, V155, P379

[9] A novel wobble rule found in starfish mitochondria - Presence of 7-methylguanosine at the anticodon wobble position expands decoding capability of tRNA [J].

Matsuyama, S ;

Ueda, T ;

Crain, PF ;

McCloskey, JA ;

Watanabe, K .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (06) :3363-3368

[10] STUDIES ON T-UTILIS TRANSFER RNATYR VARIANTS WITH ENZYMATICALLY ALTERED D-LOOP SEQUENCES .2. RELATIONSHIP BETWEEN THE TERTIARY STRUCTURE AND TYROSINE ACCEPTANCE [J].

OHYAMA, T ;

NISHIKAWA, K ;

TAKEMURA, S .

JOURNAL OF BIOCHEMISTRY, 1986, 99 (03) :859-866

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